Human MYCL1/L-Myc Antibody

Catalog # Availability Size / Price Qty
MAB40501-100
MAB40501-SP
Human  MYCL1/L‑Myc ELISA Standard Curve.
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Human MYCL1/L-Myc Antibody Summary

Species Reactivity
Human
Specificity
Detects human MYCL1/L-Myc in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 803017
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MYCL1/L‑Myc
Gly16-Asn139
Accession # P12524
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human MYCL1/L‑Myc Monoclonal Antibody (Catalog # MAB4050).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human MYCL1/L‑Myc ELISA Standard Curve. Recombinant Human MYCL1/L‑Myc protein was serially diluted 2-fold and captured by Mouse Anti-Human MYCL1/L‑Myc Monoclonal Antibody (Catalog # MAB40501) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human MYCL1/L‑Myc Monoclonal Antibody (Catalog # MAB4050) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MYCL1/L-Myc

MYCL1 is a member of the MYC family that was isolated from small cell lung carcinoma. Like the other members of the MYC family, MYCL1 is a proto-oncogene transcription factor belonging to the helix-loop-helix basic leucine zipper (HLH bzip) family. With its heterodimeric partners, MYCL1 binds to the DNA consensus site 5’ CACGTG 3’. MYCL1 is implicated in controlling a wide range of cellular processes from cellular proliferation to apoptosis.

Long Name
v-Myc Myelocytomatosis Viral Oncogene Homolog 1, Lung Carcinoma Derived
Entrez Gene IDs
4610 (Human); 16918 (Mouse); 298506 (Rat)
Alternate Names
BHLHE38; bHLHe38myc-related gene from lung cancer; Class E basic helix-loop-helix protein 38; LMyc; L-Myc; LMYCl-myc-1 proto-oncogene; MYCL; MYCL1; protein L-Myc-1; v-myc avian myelocytomatosis viral oncogene homolog 1, lung carcinoma derived; v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian)

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FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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