Human MYH7 Antibody

Catalog # Availability Size / Price Qty
MAB9096
MAB9096-SP
Detection  of Human MYH7 by Western Blot.
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Human MYH7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MYH7 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 940906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human MYH7 synthetic peptide
Accession # P12883
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human MYH7 by Western Blot. View Larger

Detection of Human MYH7 by Western Blot. Western blot shows lysates of human skeletal muscle tissue and human heart (atrium) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB9096) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018).
A specific band was detected for MYH7 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MYH7  in Human Heart. View Larger

MYH7 in Human Heart. MYH7 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB9096) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MYH7

MYH7 encodes the beta myosin heavy chain (MHC-beta ) which is a component of cardiac muscle myosin mainly expressed in the ventricle of fetal heart and represents the minority myosin in the adult heart. This is the 'slow form' of cardiac myosin as opposed to the 'fast form' (MYH6, aka MHC-alpha ) expressed more predominantely in the atria of the fetal heart and is the predominant myosin in the adult heart. The two isoforms of cardiac MHC alpha  and  beta  display 93% homology but have significantly different enzymatic properties, with  alpha  having 150-300% the contractile velocity and 60-70% actin attachment time as that of beta. Several mutations in MYH7 have been associated with inherited cardiomyopathies paraspinal and proximal muscle atrophy. MYH7 is a 223 kDa protein composed of 1935 amino acids.

Long Name
Myosin Heavy Chain 7, Cardiac Muscle, Beta
Entrez Gene IDs
4625 (Human); 140781 (Mouse); 29557 (Rat)
Alternate Names
cardiac muscle beta isoform; CMD1S; CMD1SMGC138376; CMH1; DKFZp451F047; MGC138378; MPD1; MYH7; MYHCB; MyHC-beta; myhc-slow; myopathy, distal 1; myosin heavy chain (AA 1-96); myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy polypeptide 7, cardiac muscle, beta; Myosin-7; rhabdomyosarcoma antigen MU-RMS-40.7A; SPMD; SPMM

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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