Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 639329
View all P2Y10/P2RY10 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human P2RY10
Accession # O00398

Specificity

Detects human P2RY10 in Flow Cytometry and Immunohistochemistry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human P2Y10/P2RY10 Antibody

Detection of P2Y10/P2RY10 antibody in HEK293 Human Cell Line Transfected with Human P2Y10/P2RY10 and eGFP antibody by Flow Cytometry.

Detection of P2Y10/P2RY10 in HEK293 Human Cell Line Transfected with Human P2Y10/P2RY10 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with P2Y10/P2RY10 and eGFP was stained with (A) Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) or (B) Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

P2Y10/P2RY10 antibody in Human Thymus by Immunohistochemistry (IHC-P).

P2Y10/P2RY10 in Human Thymus.

P2Y10/P2RY10 was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human P2Y10/P2RY10 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human P2Y10/P2RY10 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thymus

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: P2Y10/P2RY10

P2Y receptors are a family of purinergic G protein-coupled receptors which belong to the class A family of GPCRs. They are membrane proteins, preferentially activated by nucleotides such as ADP, ATP, UTP, UDP and UDP-glucose. This P2Y receptor is thought to be a receptor for purines. While there is little known about the activity of this specific receptor, P2Y receptors mediate various biological functions such as vasodilation, blood clotting and immune responses.

Long Name

P2Y Purinergic Receptor 10

Alternate Names

P2RY10

Entrez Gene IDs

27334 (Human); 78826 (Mouse); 317219 (Rat)

Gene Symbol

P2RY10

UniProt

O00398

Additional P2Y10/P2RY10 Products

Product Documents for Human P2Y10/P2RY10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human P2Y10/P2RY10 Antibody

For research use only

Citations for Human P2Y10/P2RY10 Antibody

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Protocols

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