Human P2Y10/P2RY10 Antibody Summary
Accession # O00398
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of P2Y10/P2RY10 in HEK293 Human Cell Line Transfected with Human P2Y10/P2RY10 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with P2Y10/P2RY10 and eGFP was stained with (A) Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) or (B) Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
P2Y10/P2RY10 in Human Thymus. P2Y10/P2RY10 was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
P2Y receptors are a family of purinergic G protein-coupled receptors which belong to the class A family of GPCRs. They are membrane proteins, preferentially activated by nucleotides such as ADP, ATP, UTP, UDP and UDP-glucose. This P2Y receptor is thought to be a receptor for purines. While there is little known about the activity of this specific receptor, P2Y receptors mediate various biological functions such as vasodilation, blood clotting and immune responses.
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Cell and Tissue Staining Kits
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