Human P2Y10/P2RY10 Antibody

Catalog # Availability Size / Price Qty
MAB10158-100
MAB10158-SP
Human P2Y10/P2RY10 Antibody in Flow Cytometry
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Human P2Y10/P2RY10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human P2RY10 in Flow Cytometry and Immunohistochemistry.
Source
Monoclonal Mouse IgG2B Clone # 639329
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human P2RY10
Accession # O00398
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of P2Y10/P2RY10  in HEK293 Human Cell Line Transfected with Human P2Y10/P2RY10 and eGFP by Flow  Cytometry. View Larger

Detection of P2Y10/P2RY10 in HEK293 Human Cell Line Transfected with Human P2Y10/P2RY10 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with P2Y10/P2RY10 and eGFP was stained with (A) Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) or (B) Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry P2Y10/P2RY10  in Human Thymus. View Larger

P2Y10/P2RY10 in Human Thymus. P2Y10/P2RY10 was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P2Y10/P2RY10

P2Y receptors are a family of purinergic G protein-coupled receptors which belong to the class A family of GPCRs. They are membrane proteins, preferentially activated by nucleotides such as ADP, ATP, UTP, UDP and UDP-glucose. This P2Y receptor is thought to be a receptor for purines. While there is little known about the activity of this specific receptor, P2Y receptors mediate various biological functions such as vasodilation, blood clotting and immune responses.

Long Name
P2Y Purinergic Receptor 10
Entrez Gene IDs
27334 (Human); 78826 (Mouse); 317219 (Rat)
Alternate Names
G-protein coupled purinergic receptor P2Y10; P2RY10; P2Y purinoceptor 10; P2Y10; P2Y10P2Y-like receptor; purinergic receptor P2Y, G-protein coupled, 10; putative P2Y purinoceptor 10

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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