Human Paxillin Antibody

Catalog # Availability Size / Price Qty
MAB4259
MAB4259-SP
Detection of Human Paxillin by Western Blot.
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Product Details
Citations (1)
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Human Paxillin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Paxillin in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 492918
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Paxillin
Asn59-Ser274
Accession # P49023
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Paxillin antibody by Western Blot. View Larger

Detection of Human Paxillin by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific diffuse band was detected for Paxillin at approximately 65 to 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Paxillin antibody in Detroit 551 Human Cell Line by Immunocytochemistry (ICC). View Larger

Paxillin in Detroit 551 Human Cell Line. Paxillin was detected in immersion fixed Detroit 551 human embryonic skin fibroblast cell line using Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Paxillin antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

Paxillin in HUVEC Human Cells. Paxillin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to actin filament tips. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Paxillin

The cytoskeletal adaptor protein Paxillin is found at the interface between actin filaments and the plasma membrane. Paxillin localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation. Increased Paxillin protein levels have also been reported in several malignancies, including renal, lung, and breast carcinomas.

Entrez Gene IDs
5829 (Human); 19303 (Mouse); 360820 (Rat)
Alternate Names
FLJ16691; Paxillin; PXN

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Citation for Human Paxillin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of lymphocyte cell-specific protein-tyrosine kinase (LCK) as a driver for invasion and migration of oral cancer by tumor heterogeneity exploitation
    Authors: J Wei beta e, J Rosemann, L Müller, M Kappler, AW Eckert, M Gla beta, D Misiak, S Hüttelmaie, WG Ballhausen, M Hatzfeld, M Haemmerle, T Gutschner
    Molecular Cancer, 2021-06-11;20(1):88.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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