Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 613643R
View all Periostin/OSF-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Periostin/OSF-2
Met1-Gln836
Accession # Q15063

Specificity

Detects human Periostin/OSF-2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Periostin/OSF‑2 Antibody

Human Periostin/OSF-2 Antibody in ELISA Standard Curve.

Human Periostin/OSF‑2 ELISA Standard Curve.

Recombinant Human Periostin/OSF-2 protein was serially diluted 2-fold and captured by Mouse Anti-Human Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB35483) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB35484) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Periostin/OSF‑2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Periostin/OSF‑2 Monoclonal Antibody (Catalog # MAB35483).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Periostin/OSF-2 DuoSet ELISA Kit (Catalog # DY3548B) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Periostin/OSF-2

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains [takeshita]. Mature human Periostin shares 91% aa sequence identity with mouse and rat Periostin. Alternative splicing generates additional isoforms with various deletions in the C-terminal region following the FAS domains. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). It is up-regulated in many carcinomas (2, 8). Periostin binds to Integrins alpha v beta 3 and alpha v beta 5 (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is up-regulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).

References

  1. Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
  2. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  3. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  4. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  7. Li, G. et al. (2006) Atherosclerosis 188:292.
  8. Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
  9. Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
  10. Erkan, M. et al. (2007) Gastroenterology 132:1447.
  11. Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
  12. Kudo, Y. et al. (2012) PLoS One 7:e44488.
  13. Snider, P. et al. (2008) Circ. Res. 102:752.
  14. Kuhn, B. et al. (2007) Nat. Med. 13:962.
  15. Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
  16. Sehra, S. et al. (2011) J. Immunol. 186:4959.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Gene Symbol

POSTN

UniProt

Q15063

Additional Periostin/OSF-2 Products

Product Documents for Human Periostin/OSF‑2 Antibody

Certificate of Analysis

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Product Specific Notices for Human Periostin/OSF‑2 Antibody

For research use only

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