Detection of Human Phospho-Caveolin‑1 (Y14) by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-Caveolin‑1 (Y14) Monoclonal Antibody (Catalog # MAB7418) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Caveolin‑1 (Y14) at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-Caveolin-1 (Y14) in MCF‑7 Human Cell Line.
Caveolin‑1 phosphorylated at Y14 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Phospho-Caveolin‑1 (Y14) Monoclonal Antibody (Catalog # MAB7418) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% aa sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.
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