Human Phospho-CD117/c-kit (Y730) Antibody

Catalog # Availability Size / Price Qty
AF3527
AF3527-SP
Detection of Human Phospho-CD117/ c‑kit (Y730) by Western Blot.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (3)

Human Phospho-CD117/c-kit (Y730) Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse CD117/c-kit when phosphorylated at Y730.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human CD117/c-kit Y730 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13-15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-CD117/ c‑kit (Y730) by Western Blot. View Larger

Detection of Human Phospho-CD117/ c‑kit (Y730) by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-CD117/c-kit (Y730) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3527), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CD117/c-kit (Y730) at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human CD117/c-kit by Simple Western<sup>TM</sup>. View Larger

Detection of Human CD117/c-kit by Simple WesternTM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 177 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-CD117/c-kit (Y730) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3527). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. The human stem cell factor receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment and a 429 aa residue cytoplasmic domain. Stem cell factor receptor is a member of the type III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF receptor is expressed in hematopoietic progenitor cells, normal B- and T-lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung and gut. In addition, SCF receptor expression has also been reported in a number of human tumor cell lines. SCF receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF receptor binds SCF with high affinity and is a potent SCF antagonist.

References
  1. Broudy, V. (1997) Blood 90:1345.
  2. Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.
Long Name
Stem Cell Factor Receptor
Entrez Gene IDs
3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Citations for Human Phospho-CD117/c-kit (Y730) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Transmembrane stem cell factor protein therapeutics enhance revascularization in ischemia without mast cell activation
    Authors: E Takematsu, M Massidda, J Auster, PC Chen, B Im, S Srinath, S Canga, A Singh, M Majid, M Sherman, A Dunn, A Graham, P Martin, AB Baker
    Nature Communications, 2022;13(1):2497.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Engineering Stem Cell Factor Ligands with Different c-Kit Agonistic Potencies
    Authors: T Tilayov, T Hingaly, Y Greenshpan, S Cohen, B Akabayov, R Gazit, N Papo
    Molecules, 2020;25(20):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human Phospho-CD117/c-kit (Y730) Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human Phospho-CD117/c-kit (Y730) Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Phospho-CD117/c-kit (Y730) Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Phospho-CD117/c-kit (Y730) Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human