Human Phospho-GSK‑3 beta (S9) Antibody

Name: Human Phospho-GSK‑3 beta (S9) Antibody
Brand: R&D Systems
SKU: MAB8934
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # MAB8934

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Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1253C

View all GSK-3 beta Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human GSK‑3 beta S9 site

Specificity

Detects human GSK‑3 beta when phosphorylated at S9 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-GSK‑3 beta (S9) Antibody

Detection of Human Phospho-GSK‑3 beta (S9) by Western Blot.

Western blot shows lysates of untreated (-) or MCF-7 human breast cancer cell line treated (+) with 100 ng/ml Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes and HeLa human cervical epithelial carcinoma cell line treated (+) with 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-GSK-3 beta Monoclonal Antibody (Catalog # MAB8934) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-GSK-3 beta (S9) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-GSK-3 beta (S9) in MCF‑7 Human Cell Line.

GSK-3 beta phosphorylated at S9 was detected in immersion fixed MCF-7 human breast cancer cell line untreated (lower panel) or treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1; upper panel) using Rabbit Anti-Human Phospho-GSK-3 beta Monoclonal Antibody (Catalog # MAB8934) at a 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Phospho-GSK‑3 beta (S9) by Simple Western^TM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-GSK-3 beta (S9) at approximately 56 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-GSK-3 beta Monoclonal Antibody (Catalog # MAB8934). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Phospho-GSK‑3 beta (S9) Antibody

Application

Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1)

Simple Western

5 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1)

Western Blot

0.5 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1) and HeLa human cervical epithelial carcinoma cell line treated with PMA

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified asan inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.