Human Phospho-GSK-3 beta (S9) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8934
MAB8934-SP
Detection of Human Phospho-GSK‑3 beta  (S9) by Western Blot.
3 Images
Product Details
FAQs
Supplemental Products
Reviews

Human Phospho-GSK-3 beta (S9) Antibody Summary

Species Reactivity
Human
Specificity
Detects human GSK‑3 beta when phosphorylated at S9 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1253C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human GSK‑3 beta S9 site
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
Simple Western
1:100 dilution
See below
Immunocytochemistry
1:150-1:300 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-GSK‑3 beta  (S9) by Western Blot. View Larger

Detection of Human Phospho-GSK‑3 beta (S9) by Western Blot. Western blot shows lysates of untreated (-) or MCF‑7 human breast cancer cell line treated (+) with 100 ng/ml Recombinant Human IGF‑I (Catalog # 291-G1) for 60 minutes and HeLa human cervical epithelial carcinoma cell line treated (+) with 200 nM PMA for 20 minutes. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Phospho-GSK‑3 beta Monoclonal Antibody (Catalog # MAB8934) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-GSK‑3 beta (S9) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-GSK-3 beta  (S9) in MCF‑7 Human Cell Line. View Larger

Phospho-GSK-3 beta (S9) in MCF‑7 Human Cell Line. GSK‑3 beta phosphorylated at S9 was detected in immersion fixed MCF‑7 human breast cancer cell line untreated (lower panel) or treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1; upper panel) using Rabbit Anti-Human Phospho-GSK‑3 beta Monoclonal Antibody (Catalog # MAB8934) at a 1:250 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human Phospho-GSK‑3 beta  (S9) by Simple WesternTM. View Larger

Detection of Human Phospho-GSK‑3 beta (S9) by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 60 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-GSK‑3 beta (S9) at approximately 56 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human Phospho-GSK‑3 beta Monoclonal Antibody (Catalog # MAB8934). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified asan inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Long Name
Glycogen Synthase Kinase 3 beta
Entrez Gene IDs
2932 (Human); 56637 (Mouse); 84027 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Secondary Antibodies

Reviews for Human Phospho-GSK-3 beta (S9) Antibody

There are currently no reviews for this product. Be the first to review Human Phospho-GSK-3 beta (S9) Antibody and earn rewards!

Have you used Human Phospho-GSK-3 beta (S9) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review