Human Phospho-STAT3 (S727) Antibody

Name: Human Phospho-STAT3 (S727) Antibody
Brand: R&D Systems
SKU: AF4934
Price: 429 USD
Availability: InStock

R&D Systems | Catalog # AF4934

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Citations (2)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all STAT3 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human STAT3 S727 site
Accession # P40763

Specificity

Detects human STAT3 when phosphorylated at S727 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-STAT3 (S727) Antibody

Detection of Human Phospho-STAT3 (S727) by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-STAT3 (S727) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4934) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT3 (S727) was detected at approximately 110 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human STAT3 Monoclonal Antibody (lower panel, Catalog # MAB1799). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-STAT3 (S727) Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human IL‑6 (Catalog # 206-IL)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: STAT3

PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator. Mature mouse PI16 is a type I transmembrane protein 469 amino acids (aa) in length. The extracellular region includes all but the C‑terminal 21 amino acids. A soluble form of PI16 is known. Although its predicted MW is 50 kDa, it runs anomalously at 70 kDa (unmodified) in SDS‑PAGE. With glycosylation, it appears to be 100‑110 kDa in size. Three potential variants exist. One shows a deletion of aa 51‑124, a second shows a 9 aa extension at the N‑terminus, and a third shows a deletion of aa 191‑452. Over aa 1‑464, mouse PI16 shares 86% and 61% aa identity with rat and human PI16, respectively.

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)