Human Phospho-Vav-1 (Y160) Antibody

Detection of Human Phospho-Vav‑1 (Y160) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes and 10 µg/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) for 15 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Vav-1 (Y160) Monoclonal Antibody (Catalog # MAB37861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Vav-1 (Y160) at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-Vav-1 (Y160) in Jurkat Human Cell Line. Vav-1 phosphorylated at Y160 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Vav-1 (Y160) Monoclonal Antibody (Catalog # MAB37861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human Phospho-Vav‑1 (Y160) by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Human Phospho-Vav‑1 (Y160) at approximately 116 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Phospho-Vav‑1 (Y160) Monoclonal Antibody (Catalog # MAB37861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.