Human Phospho-Vav-1 (Y160) Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human VAV-1 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 751411
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Phosphopeptide containing the human Vav-1 Y160 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human Phospho-Vav‑1 (Y160) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes and 10 µg/mL Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) for 15 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Vav‑1 (Y160) Monoclonal Antibody (Catalog # MAB37861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Vav‑1 (Y160) at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Phospho-Vav-1 (Y160) in Jurkat Human Cell Line. Vav‑1 phosphorylated at Y160 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Vav‑1 (Y160) Monoclonal Antibody (Catalog # MAB37861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human Phospho-Vav‑1 (Y160) by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Human Phospho-Vav‑1 (Y160) at approximately 116 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human
Phospho-Vav‑1 (Y160) Monoclonal Antibody (Catalog # MAB37861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vav-1
The Vav-1 proto-oncogene is a 95 kDa guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 is a cytosolic protein that is primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells. It promotes intracellular signaling by its multiple protein binding motifs. Vav-1 is phosphorylated on Y160 after cell adhesion via integrin avb3. This phosphorylation is necessary for adhesion‑mediated Rho activation and association between avb3 and Vav-1. The peptide immunogen represents a sequence that is conserved between human, mouse and rat Vav-1.
  • Entrez Gene IDs:
    7409 (Human); 22324 (Mouse); 25156 (Rat)
  • Alternate Names:
    oncogene vav; proto-oncogene vav; vav 1 guanine nucleotide exchange factor; vav proto-oncogene; Vav1; Vav-1; vav-T; VAVvav 1 oncogene
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Distinct structural and catalytic roles for Zap70 in formation of the immunological synapse in CTL.
    Authors: Jenkins, Misty R, Stinchcombe, Jane C, Au-Yeung, Byron B, Asano, Yukako, Ritter, Alex T, Weiss, Arthur, Griffiths, Gillian
    Elife, 2014;3(0):e01310.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
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Mouse IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2A PE-conjugated Antibody

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