Human/Primate CXCL16 Antibody

Catalog # Availability Size / Price Qty
AF976
AF976-SP
Chemotaxis Induced by CXCL16 and Neutralization by Human CXCL16 Antibody.
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Product Details
Citations (10)
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Human/Primate CXCL16 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human CXCL16 in ELISAs and Western blots. In ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) CXCL16, rm6Ckine, recombinant human (rh) BLC, rhCTACK, rhIL-8, rhPF4, and rhLymphotactin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CXCL16
Asn49-Pro137
Accession # NP_071342
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX)
Flow Cytometry
0.25 µg/106 cells
Human monocyte-derived dendritic cells
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human/Primate CXCL16 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate CXCL16 Biotinylated Antibody (Catalog # BAF976)

Standard: Recombinant Human CXCL16 (Histidine-tagged) Protein (Catalog # 1164-CX)

Neutralization
Measured by its ability to neutralize CXCL16-induced chemotaxis in BaF3 mouse pro‑B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nat. Immunol.The Neutralization Dose (ND50) is typically
0.05-0.25 μg/mL in the presence of 20 ng/mL Recombinant Human CXCL16 Chemokine Domain.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Chemotaxis Induced by CXCL16 and Neutralization by Human CXCL16 Antibody. View Larger

Chemotaxis Induced by CXCL16 and Neutralization by Human CXCL16 Antibody. Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX) induces chemotaxis in BaF3 mouse pro‑B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human CXCL16 Chemokine Domain (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976). The ND50 is typically
0.05-0.25 μg/mL.

Immunohistochemistry CXCL16 in Human Lymph Node. View Larger

CXCL16 in Human Lymph Node. CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry CXCL16 in Human Lymphoma. View Larger

CXCL16 in Human Lymphoma. CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL16

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Together with Fractalkine (CX3CL1), CXCL16 constitute the only two transmembrane chemokines within the superfamily. The gene for human CXCL16 predicts a 273 amino acid (aa) residue precursor protein with a putative signal peptide, a CXC chemokine domain, a mucin-like spacer region, a transmembrane domain and a cytoplasmic domain with a potential tyrosine phosphorylation and SH2 protein-binding site. Mouse and human CXCL16 share 70% aa sequence similarity within their chemokine domains and 49% overall aa sequence identity. By northern blot analysis, CXCL16 expression is detected in various human organs except for brain, bone marrow, skeletal muscle or colon. By flow cytometry, CXCL16 has been detected on the surface CD19+ B cells, CD14+ monocytes/macrophages, and CD11c+ splenic and lymph node dendritic cells. Functional CXCL16 can be shed from the cell surface as an approximately 35 kDa soluble protein. The functional receptor for CXCL16 has been identified as CXCR6 (also known as Bonzo, STRL33 or TYMSTR), a receptor previously shown to be a co-receptor for HIV entry. CXCL16 has also been independently cloned and named SRPSOX (scavenger receptor that binds phosphatidylserine and oxidized lipoprotein). It was shown to be a specific receptor for OxLDL but not LDL or acetyl-LDL.

References
  1. Matloubian, M. et al. (2000) Nature Immun. 1:298.
  2. Shimaoka, T. et al. (2000) J. Biol. Chem. 275:40663.
  3. Wilbanks, A. et al. (2001) J. Immun. 166:5145.
Entrez Gene IDs
58191 (Human); 66102 (Mouse)
Alternate Names
chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16

Product Datasheets

Citations for Human/Primate CXCL16 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease
    Authors: X Qu, X Dang, W Wang, Y Li, D Xu, D Shang, Y Chang
    Mediators Inflamm., 2018;2018(0):7501851.
    Applications: Neutralization
  2. Chemokine CXCL16 mediates acinar cell necrosis in cerulein induced acute pancreatitis in mice
    Authors: Y Sakuma, Y Kodama, T Eguchi, N Uza, Y Tsuji, M Shiokawa, T Maruno, K Kuriyama, Y Nishikawa, Y Yamauchi, M Tsuda, T Ueda, T Matsumori, T Morita, T Tomono, N Kakiuchi, A Mima, Y Sogabe, S Marui, T Kuwada, A Okada, T Watanabe, H Nakase, T Chiba, H Seno
    Sci Rep, 2018;8(1):8829.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro.
    Authors: Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang
    PLoS ONE, 2014;9(6):e99056.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
    Authors: Dieudonne A, Torres D, Blanchard S, Taront S, Jeannin P, Delneste Y, Pichavant M, Trottein F, Gosset P
    PLoS ONE, 2012;7(8):e41952.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas.
    Authors: Held-Feindt J, Rehmke B, Mentlein R, Hattermann K, Knerlich F, Hugo HH, Ludwig A, Mehdorn HM
    Glia, 2008;56(7):764-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. CXCR6-CXCL16 interaction in the pathogenesis of Juvenile Idiopathic Arthritis.
    Authors: Martini G, Cabrelle A, Calabrese F, Carraro S, Scquizzato E, Teramo A, Facco M, Zulian F, Agostini C
    Clin. Immunol., 2008;129(2):268-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma.
    Authors: Wente MN, Gaida MM, Mayer C, Michalski CW, Haag N, Giese T, Felix K, Bergmann F, Giese NA, Friess H
    Int. J. Oncol., 2008;33(2):297-308.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. CXCL16-mediated cell recruitment to rheumatoid arthritis synovial tissue and murine lymph nodes is dependent upon the MAPK pathway.
    Authors: Ruth JH, Haas CS, Park CC, Amin MA, Martinez RJ, Haines GK, Shahrara S, Campbell PL, Koch AE
    Arthritis Rheum., 2006;54(3):765-78.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
    Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
    Arthritis Rheum., 2006;54(8):2402-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. EPHB4 regulates chemokine-evoked trophoblast responses: a mechanism for incorporating the human placenta into the maternal circulation.
    Authors: Red-Horse K, Kapidzic M, Zhou Y, Feng KT, Singh H, Fisher SJ
    Development, 2005;132(18):4097-106.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Human/Primate CXCL16 Antibody
By Anonymous on 06/16/2017
Application: ICC/IF Sample Tested: OVARIAN CELL LINE,OVCAR5 CELL LINE Species: Human