Human/Primate CXCL16 Antibody

(8 citations)
(1 Review)
  
  • Species Reactivity
    Human, Primate
  • Specificity
    Detects human CXCL16 in ELISAs and Western blots. In ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) CXCL16, rm6Ckine, recombinant human (rh) BLC, rhCTACK, rhIL-8, rhPF4, and rhLymphotactin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CXCL16
    Asn49-Pro137
    Accession # NP_071342
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX)
  • Flow Cytometry
    0.25 µg/106 cells
    Human monocyte-derived dendritic cells
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human/Primate CXCL16 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human/Primate CXCL16 Antibody (Catalog # AF976)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Primate CXCL16 Biotinylated Antibody (Catalog # BAF976)
  • ELISA Standard
     
    Recombinant Human CXCL16 (Histidine-tagged) Protein (Catalog # 1164-CX)
  • Neutralization
    Measured by its ability to neutralize CXCL16-induced chemotaxis in BaF3 mouse pro‑B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nat. Immunol.The Neutralization Dose (ND50) is typically
    0.05-0.25 μg/mL in the presence of 20 ng/mL Recombinant Human CXCL16 Chemokine Domain.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL16 and Neutralization by Human CXCL16 Antibody. Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX) induces chemotaxis in BaF3 mouse pro‑B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human CXCL16 Chemokine Domain (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976). The ND50 is typically
0.05-0.25 μg/mL.
Immunohistochemistry
CXCL16 in Human Lymph Node. CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
CXCL16 in Human Lymphoma. CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL16

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Together with Fractalkine (CX3CL1), CXCL16 constitute the only two transmembrane chemokines within the superfamily. The gene for human CXCL16 predicts a 273 amino acid (aa) residue precursor protein with a putative signal peptide, a CXC chemokine domain, a mucin-like spacer region, a transmembrane domain and a cytoplasmic domain with a potential tyrosine phosphorylation and SH2 protein-binding site. Mouse and human CXCL16 share 70% aa sequence similarity within their chemokine domains and 49% overall aa sequence identity. By northern blot analysis, CXCL16 expression is detected in various human organs except for brain, bone marrow, skeletal muscle or colon. By flow cytometry, CXCL16 has been detected on the surface CD19+ B cells, CD14+ monocytes/macrophages, and CD11c+ splenic and lymph node dendritic cells. Functional CXCL16 can be shed from the cell surface as an approximately 35 kDa soluble protein. The functional receptor for CXCL16 has been identified as CXCR6 (also known as Bonzo, STRL33 or TYMSTR), a receptor previously shown to be a co-receptor for HIV entry. CXCL16 has also been independently cloned and named SRPSOX (scavenger receptor that binds phosphatidylserine and oxidized lipoprotein). It was shown to be a specific receptor for OxLDL but not LDL or acetyl-LDL.

  • References:
    1. Matloubian, M. et al. (2000) Nature Immun. 1:298.
    2. Shimaoka, T. et al. (2000) J. Biol. Chem. 275:40663.
    3. Wilbanks, A. et al. (2001) J. Immun. 166:5145.
  • Entrez Gene IDs:
    58191 (Human); 66102 (Mouse)
  • Alternate Names:
    chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro.
    Authors: Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang
    PLoS ONE, 2014;9(6):e99056.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
    Authors: Dieudonne A, Torres D, Blanchard S, Taront S, Jeannin P, Delneste Y, Pichavant M, Trottein F, Gosset P
    PLoS ONE, 2012;7(8):e41952.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas.
    Authors: Held-Feindt J, Rehmke B, Mentlein R, Hattermann K, Knerlich F, Hugo HH, Ludwig A, Mehdorn HM
    Glia, 2008;56(7):764-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. CXCR6-CXCL16 interaction in the pathogenesis of Juvenile Idiopathic Arthritis.
    Authors: Martini G, Cabrelle A, Calabrese F, Carraro S, Scquizzato E, Teramo A, Facco M, Zulian F, Agostini C
    Clin. Immunol., 2008;129(2):268-76.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma.
    Authors: Wente MN, Gaida MM, Mayer C, Michalski CW, Haag N, Giese T, Felix K, Bergmann F, Giese NA, Friess H
    Int. J. Oncol., 2008;33(2):297-308.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. CXCL16-mediated cell recruitment to rheumatoid arthritis synovial tissue and murine lymph nodes is dependent upon the MAPK pathway.
    Authors: Ruth JH, Haas CS, Park CC, Amin MA, Martinez RJ, Haines GK, Shahrara S, Campbell PL, Koch AE
    Arthritis Rheum., 2006;54(3):765-78.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  7. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
    Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
    Arthritis Rheum., 2006;54(8):2402-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. EPHB4 regulates chemokine-evoked trophoblast responses: a mechanism for incorporating the human placenta into the maternal circulation.
    Authors: Red-Horse K, Kapidzic M, Zhou Y, Feng KT, Singh H, Fisher SJ
    Development, 2005;132(18):4097-106.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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WB BAF017
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human/Primate CXCL16 Antibody AF976
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 ICC/IF Human Anonymous 06/16/2017
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Immunocytochemistry/Immunofluorescence Human/Primate CXCL16 Antibody AF976
Immunocytochemistry/Immunofluorescence: Human/Primate CXCL16 Antibody [AF976]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedOVARIAN CELL LINE,OVCAR5 CELL LINE
SpeciesHuman

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