Human Progranulin/PGRN Antibody Summary
Accession # P28799
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse Progranulin/PGRN by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Progranulin/PGRN Polyclonal Antibody (Catalog # MAB24201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Progranulin/PGRN at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. For Western blotting, Catalog # AF2420is recommended.
Progranulin/PGRN in Human Breast Cancer Tissue. Progranulin/PGRN was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Progranulin/PGRN Polyclonal Antibody (Catalog # MAB24201) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Progranulin, also known as Acrogranin, PC Cell-derived Growth Factor (PCDGF) and Epithelin/Granulin Precursor, is a ubiquitously expressed, 88 kDa, secreted glycoprotein (1‑3). Structurally, it does not belong to any of the well-established growth factor families (4). Human Progranulin is 593 amino acids (aa) in length and contains a 17 aa signal sequence and 5 potential sites for N-linked glycosylation (Swiss Prot # P28799). It has a highly repetitive organization, containing seven tandem copies of a 55-57 aa consensus motif that contains 12 conserved cysteine residues: VXCX5-6CX5CCX8CCX6CCXDX2HCCPX4CX5-6CX2 (1). There is one alternate splice form for human Progranulin. This has a deletion of aa corresponding to aa 377-531 of the standard form. Progranulin is secreted as a full length form (2, 4), and may undergo proteolysis leading to the release of numerous peptides made from the seven tandem repeats, called the granulins (5-7). Human Progranulin shares 75% aa sequence identity with mouse and rat Progranulin. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development, and pathological processes (4). It has been isolated as a differentially expressed gene during mesothelial differentiation (8), macrophage development (9), the development of rheumatoid arthritis and osteoarthritis (10), sexual differentiation of the brain (11), and has also been shown to be a mediator of cartilage proliferation and of wound response and tissue repair (4, 12 13). High levels of Progranulin expression have been found to be associated with several human cancers and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma, and multiple myeloma (4-5, 12, 14-19). In addition, mutations in the Progranulin gene are a cause of frontotemporal dementia, and increased expression of Progranulin is seen in activated microglia in many neurodegenerative diseases including Creutzfeldt-Jakob disease, motor neuron disease and Alzheimer's disease (20). Mutations in Progranulin causing neurodegenerative disease indicate that Progranulin is important for neuronal survival (20).
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