Human PTPN14 Antibody MAB4458: R&D Systems

Human PTPN14 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human PTPN14 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PTPN13 or rhPTPN22.
  • Source
    Monoclonal Mouse IgG2B Clone # 448701
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PTPN14
    Leu329-Leu900
    Accession # Q15678.2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PTPN14 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, HT‑29 human colon adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPN14 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
PTPN14 in Human Placenta. PTPN14 was detected in immersion fixed paraffin-embedded sections of human placenta using Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTPN14

Protein Tyrosine Phosphatase, Non-receptor type 14 (PTPN14), also called Phosphatase with Ezrin Domain (PEZ) and PTP36, is a 135 kDa cytosolic protein that dephosphorylates tyrosine residues in proteins. PTPN14’s Ezrin domain is homologous to erythrocyte Band 4.1 protein, targeting the phosphatase to cytoskeletal elements such as actin. PTPN14 associates with beta-catenin in adhesion zones in cells that are touching, but is nuclear in isolated, proliferating cells. Loss-of-function mutations in PTPN14 are found in 26% of colorectal cancers, suggesting it is a tumor suppressor.

  • Long Name:
    Protein Tyrosine Phosphatase Non-receptor Type 14
  • Entrez Gene IDs:
    5784 (Human); 19250 (Mouse); 305064 (Rat)
  • Alternate Names:
    EC 3.1.3; EC 3.1.3.48; MGC126803; PEZ; PEZcytoskeletal-associated protein tyrosine phosphatase; protein tyrosine phosphatase, non-receptor type 14; Protein-tyrosine phosphatase pez; PTP36; PTPN14; tyrosine-protein phosphatase non-receptor type 14
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Protein-tyrosine Phosphatase and Kinase Specificity in Regulation of SRC and Breast Tumor Kinase.
    Authors: Fan G, Aleem S, Yang M, Miller W, Tonks N
    J Biol Chem, 2015;290(26):15934-47.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. A novel phosphatidic acid-protein-tyrosine phosphatase D2 axis is essential for ERBB2 signaling in mammary epithelial cells.
    Authors: Ramesh M, Krishnan N, Muthuswamy S, Tonks N
    J Biol Chem, 2015;290(15):9646-59.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Protein-tyrosine phosphatase 1B antagonized signaling by insulin-like growth factor-1 receptor and kinase BRK/PTK6 in ovarian cancer cells.
    Authors: Fan G, Lin G, Lucito R, Tonks N
    J Biol Chem, 2013;288(34):24923-34.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. YAP modifies cancer cell sensitivity to EGFR and survivin inhibitors and is negatively regulated by the non-receptor type protein tyrosine phosphatase 14.
    Authors: Huang JM, Nagatomo I
    2012;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Isotype Controls
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Secondary Antibodies
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Mouse IgG Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse IgG2B Antibody

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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