Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 599542
View all DYRK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DYRK2
aa 1-107
Accession # NP_003574

Specificity

Detects human and rat DYRK2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Rat DYRK2 Antibody

Detection of Human and Rat DYRK2 antibody by Western Blot.

Detection of Human and Rat DYRK2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat DYRK2 Monoclonal Antibody (Catalog # MAB5408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DYRK2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Rat DYRK2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded testis

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, CCD‑1070Sk human foreskin fibroblast cell line, and L6 rat myoblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB5408 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DYRK2

DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a 58‑62 kDa member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. It is expressed in testis and shows dual substrate specificity; autophosphorylation on Tyr382 to self‑activate, and a Ser/Thr phosphorylation of target molecules. Substrates include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 initiates cell apoptosis. Human DYRK2 is 601 amino acids (aa) in length. It contains one kinase catalytic domain (aa 222‑535). One isoform variant shows an alternate start site at Met74. This shorter isoform was used for immunization. Thus, over aa 1‑107 (or aa 74‑180 of the long form), human DYRK2 shares 93% aa identity with mouse DYRK2.

Long Name

Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 2

Alternate Names

dual specificity tyrosine-phosphorylation-regulated kinase 2, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2, EC 2.7.12, EC 2.7.12.1, FLJ21217, FLJ21365

Entrez Gene IDs

8445 (Human); 69181 (Mouse); 314862 (Rat)

Gene Symbol

DYRK2

UniProt

NP_003574

Additional DYRK2 Products

Product Documents for Human/Rat DYRK2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat DYRK2 Antibody

For research use only

Related Research Areas

Intracellular Kinases

Customer Reviews for Human/Rat DYRK2 Antibody (1)

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  • Human/Rat DYRK2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HepG2 human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 07/07/2022
    Human/Rat DYRK2 Antibody MAB5408

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Protocols

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