Human/Rat DYRK2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Rat DYRK2 by Western Blot.
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Human/Rat DYRK2 Antibody Summary

Species Reactivity
Human, Rat
Detects human and rat DYRK2 in Western blots.
Monoclonal Mouse IgG2B Clone # 599542
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human DYRK2
aa 1-107
Accession # NP_003574
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
8-25 µg/mL
Immersion fixed paraffin-embedded testis

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat DYRK2 antibody by Western Blot. View Larger

Detection of Human and Rat DYRK2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat DYRK2 Monoclonal Antibody (Catalog # MAB5408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DYRK2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DYRK2

DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a 58‑62 kDa member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. It is expressed in testis and shows dual substrate specificity; autophosphorylation on Tyr382 to self‑activate, and a Ser/Thr phosphorylation of target molecules. Substrates include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 initiates cell apoptosis. Human DYRK2 is 601 amino acids (aa) in length. It contains one kinase catalytic domain (aa 222‑535). One isoform variant shows an alternate start site at Met74. This shorter isoform was used for immunization. Thus, over aa 1‑107 (or aa 74‑180 of the long form), human DYRK2 shares 93% aa identity with mouse DYRK2.

Long Name
Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 2
Entrez Gene IDs
8445 (Human); 69181 (Mouse); 314862 (Rat)
Alternate Names
dual specificity tyrosine-phosphorylation-regulated kinase 2; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2; DYRK2; EC 2.7.12; EC; FLJ21217; FLJ21365

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human/Rat DYRK2 Antibody
By Anonymous on 07/07/2022
Application: WB Sample Tested: HepG2 human hepatocellular carcinoma cell line Species: Human