Human/Rat DYRK2 Antibody Summary
Accession # NP_003574
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Rat DYRK2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat DYRK2 Monoclonal Antibody (Catalog # MAB5408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DYRK2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a 58‑62 kDa member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. It is expressed in testis and shows dual substrate specificity; autophosphorylation on Tyr382 to self‑activate, and a Ser/Thr phosphorylation of target molecules. Substrates include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 initiates cell apoptosis. Human DYRK2 is 601 amino acids (aa) in length. It contains one kinase catalytic domain (aa 222‑535). One isoform variant shows an alternate start site at Met74. This shorter isoform was used for immunization. Thus, over aa 1‑107 (or aa 74‑180 of the long form), human DYRK2 shares 93% aa identity with mouse DYRK2.
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