Human S100P Antibody

Catalog # Availability Size / Price Qty
AF2957
AF2957-SP
Detection of Human S100P by Western Blot.
3 Images
Product Details
Citations (5)
FAQs
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Human S100P Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100P in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) S100B and rhS100A10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human S100P
Met1-Lys95
Accession # P25815
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
0.3-15 µg/mL
See below
Knockout Validated
S100P is specifically detected in HT‑29 human colon adenocarcinoma parental cell line but is not detectable in S100P knockout HT‑29 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human S100P antibody by Western Blot. View Larger

Detection of Human S100P by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma parental cell line and S100P knockout HT-29 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100P at approximately 10 kDa (as indicated) in the parental HT-29 cell line, but is not detectable in knockout HT-29 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry S100P antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

S100P in Human Stomach. S100P was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Knockout Validated S100P Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line. View Larger

S100P Specificity is Shown by Immunocytochemistry in Knockout Cell Line. S100P was detected in immersion fixed HT-29 human colon adenocarcinoma cell line but is not detected in S100P knockout (KO) HT-29 Human Cell Line cell line using Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100P

S100P is a member of the S100 protein family, whose members are calcium-binding proteins containing two characteristic E-F hand motifs. S100P proteins exist as homodimers or heterodimers (e.g. with S100A1). They interact with target proteins in a Ca++-dependent manner to regulate cell functions. S100P is localized intracellularly but can also be released from cells to act extracellularly. The amino acid sequence of human S100P is 47% identical to that of rat S100P.

Long Name
S100 Calcium Binding Protein P
Entrez Gene IDs
6286 (Human)
Alternate Names
MIG9; migration-inducing gene 9; Protein S100-E; S100 calcium binding protein P; S100 calcium-binding protein Pprotein S100-P; S100E; S100P

Product Datasheets

Citations for Human S100P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. An immunostaining panel of C-reactive protein, N-cadherin, and S100P is useful for intrahepatic cholangiocarcinoma subtyping
    Authors: M Akita, R Sawada, M Komatsu, N Suleman, T Itoh, T Ajiki, N Heaton, T Fukumoto, Y Zen
    Human pathology, 2020;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Morphological subclassification of intrahepatic cholangiocarcinoma: etiological, clinicopathological, and molecular features.
    Authors: Liau J, Tsai J, Yuan R, Chang C, Lee H, Jeng Y
    Mod Pathol, 2014;27(8):1163-73.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein).
    Authors: Shimamoto, Seiko, Kubota, Yasuo, Yamaguchi, Fuminori, Tokumitsu, Hiroshi, Kobayashi, Ryoji
    J Biol Chem, 2013;288(10):7158-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  4. Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma.
    Authors: Bartling B, Rehbein G, Simm A, Silber RE, Hofmann HS
    Int. J. Oncol., 2010;36(4):1015-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Identification and characterization of novel ERC-55 interacting proteins: evidence for the existence of several ERC-55 splicing variants; including the cytosolic ERC-55-C.
    Authors: Ludvigsen M, Jacobsen C, Maunsbach AB, Honore B
    Proteomics, 2009;9(23):5267-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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