Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all S100P Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100P
Met1-Lys95
Accession # P25815

Specificity

Detects human S100P in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) S100B and rhS100A10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human S100P Antibody

Detection of Human S100P antibody by Western Blot.

Detection of Human S100P by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma parental cell line and S100P knockout HT-29 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100P at approximately 10 kDa (as indicated) in the parental HT-29 cell line, but is not detectable in knockout HT-29 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

S100P antibody in Human Stomach by Immunohistochemistry (IHC-P).

S100P in Human Stomach.

S100P was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

S100P Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line.

S100P Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

S100P was detected in immersion fixed HT-29 human colon adenocarcinoma cell line but is not detected in S100P knockout (KO) HT-29 Human Cell Line cell line using Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human S100P Antibody

Application
Recommended Usage

Immunohistochemistry

0.3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human normal liver, stomach, and prostate cancer tissue

Knockout Validated

S100P is specifically detected in HT‑29 human colon adenocarcinoma parental cell line but is not detectable in S100P knockout HT‑29 cell line.

Western Blot

0.5 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100P

S100P is a member of the S100 protein family, whose members are calcium-binding proteins containing two characteristic E-F hand motifs. S100P proteins exist as homodimers or heterodimers (e.g. with S100A1). They interact with target proteins in a Ca++-dependent manner to regulate cell functions. S100P is localized intracellularly but can also be released from cells to act extracellularly. The amino acid sequence of human S100P is 47% identical to that of rat S100P.

Long Name

S100 Calcium Binding Protein P

Alternate Names

MIG9, migration-inducing gene 9, Protein S100-E, S100 calcium binding protein P, S100 calcium-binding protein Pprotein S100-P, S100E

Entrez Gene IDs

6286 (Human)

Gene Symbol

S100P

UniProt

P25815

Additional S100P Products

Product Documents for Human S100P Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100P Antibody

For research use only

Citations for Human S100P Antibody

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Protocols

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