hh Human S100P Antibody AF2957: R&D Systems

Human S100P Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human S100P in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) S100B and rhS100A10 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human S100P
    Met1-Lys95
    Accession # P25815
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Knockout Validated
    S100P is specifically detected in HT‑29 human colon adenocarcinoma parental cell line but is not detectable in S100P knockout HT‑29 cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human S100P by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma parental cell line and S100P knockout HT‑29 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100P at approximately 10 kDa (as indicated) in the parental HT‑29cell line, but is not detectable in knockout HT‑29 cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
S100P in Human Stomach Cancer Tissue. S100P was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100P
S100P is a member of the S100 protein family, whose members are calcium-binding proteins containing two characteristic E-F hand motifs. S100P proteins exist as homodimers or heterodimers (e.g. with S100A1). They interact with target proteins in a Ca++-dependent manner to regulate cell functions. S100P is localized intracellularly but can also be released from cells to act extracellularly. The amino acid sequence of human S100P is 47% identical to that of rat S100P.
  • Long Name:
    S100 Calcium Binding Protein P
  • Entrez Gene IDs:
    6286 (Human)
  • Alternate Names:
    MIG9; migration-inducing gene 9; Protein S100-E; S100 calcium binding protein P; S100 calcium-binding protein Pprotein S100-P; S100E; S100P
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Morphological subclassification of intrahepatic cholangiocarcinoma: etiological, clinicopathological, and molecular features.
    Authors: Liau J, Tsai J, Yuan R, Chang C, Lee H, Jeng Y
    Mod Pathol, 2014;27(8):1163-73.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein).
    Authors: Shimamoto, Seiko, Kubota, Yasuo, Yamaguchi, Fuminori, Tokumitsu, Hiroshi, Kobayashi, Ryoji
    J Biol Chem, 2013;288(10):7158-68.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  3. Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma.
    Authors: Bartling B, Rehbein G, Simm A, Silber RE, Hofmann HS
    Int. J. Oncol., 2010;36(4):1015-21.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Identification and characterization of novel ERC-55 interacting proteins: evidence for the existence of several ERC-55 splicing variants; including the cytosolic ERC-55-C.
    Authors: Ludvigsen M, Jacobsen C, Maunsbach AB, Honore B
    Proteomics, 2009;9(23):5267-87.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Normal Goat IgG Control

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