Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1032714
View all Tau Primary Antibodies »

Product Specifications

Immunogen

Human Tau H synthetic peptide
Accession # P10636

Specificity

Detects Human Tau in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Tau Antibody

Tau in Human Brain (Alzheimer's).

Tau was detected in immersion fixed paraffin-embedded sections of human brain (Alzheimer's) using Mouse Anti-Human Tau Monoclonal Antibody (Catalog # MAB10629) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Tau Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (Alzheimer's)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tau

Tau protein is named for its ability to serve as a bridge for microtubule formation and stabilization. Tau in PNS neurons is 758 amino acids (aa) in length and 110 kDa in size. It migrates anomalously in SDS-PAGE. Its N-terminus binds plasma membrane components while its C‑terminus binds microtubules. The microtubule-binding region contains four consecutive Tau/MAP repeats between aa 561‑685. Each repeat is 30 aa long, Ser/Thr rich, and shows a PGGG motif. Multiple isoforms exist, ranging from 316 to 776 aa in length. Various deletions occur within the first 500 aa, with some isoforms also showing an absence of the second Tau repeat (aa 592‑622). Between aa 624‑756, human Tau protein shows absolute identity to mouse and dog Tau.

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Entrez Gene IDs

4137 (Human); 17762 (Mouse); 29477 (Rat)

Gene Symbol

MAPT

UniProt

P10636

Additional Tau Products

Product Documents for Human Tau Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Tau Antibody

For research use only

Citations for Human Tau Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human Tau Antibody

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  • Q: Which isoform of Tau does Human Tau Antibody, Catalog # MAB10629, clone 1032714, detect?

    A: This anitbody was raised against a Tau sythetic protein. Our testing supports detection of Tau381, Catalog # SP-498. We have not evaluated detection against other isoforms of Tau.

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