Human TCF7L1 Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human TCF7L1 in direct ELISAs. In direct ELISAs, 5-10% cross-reactivity with recombinant human (rh) TCF-4 (aa 102-516) and rhTCF-12 is observed. No cross-reactivity with rhTCF7L1 (aa 151-320), rhTCF7L2, rhTCF-1, -2, -3, -4 (aa 241-365), rhLEF-1, or recombinant mouse TCF-1 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 622782
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant human TCF7L1
    Lys429-Ser581
    Accession # Q9HCS4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunohistochemistry
TCF7L1 in Human Brain. TCF7L1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human TCF7L1 Monoclonal Antibody (Catalog # MAB61161) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TCF7L1/TCF3

Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63 kDa member of the TCF/LEF family of proteins.  Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346-414).  Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1.  TCF7L1 is expressed in hair follicles and skin keratinocytes and at lower levels in stomach epithelium.  Functionally, it participates in the Wnt signaling pathway.  TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1 and as an activator in its presence.  TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules, and the development of the barrier function of the epidermis.

  • Long Name:
    Transcription Factor 7-like 1
  • Entrez Gene IDs:
    83439 (Human); 21415 (Mouse); 312451 (Rat)
  • Alternate Names:
    HMG box transcription factor 3; TCF3; TCF-3; TCF7L1; transcription factor 7-like 1 (T-cell specific, HMG-box); transcription factor 7-like 1
Related Research Areas

FAQs

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Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 15
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 5
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016 1
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Antigen Retrieval Reagent-Acidic

CTS014
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 34  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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