Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF‑ beta, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, and recombinant porcine TNF‑ alpha is observed.
Recombinant Monoclonal Mouse IgG1 Clone # 28401R
Protein A or G purified from cell culture supernatant
E. coli-derived recombinant human TNF-alpha Gly57-Leu233 Accession # P01375
Supplied as a solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Human TNF‑ alpha Biotinylated Antibody (Catalog # BAF210)
Measured by its ability to neutralize TNF-alpha -induced cytotoxicity in the L-929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.01-0.04 μg/mL in the presence of 0.75 ng/mL Recombinant Human TNF-alpha and 1 μg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cytotoxicity Induced by TNF-alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Recombinant Monoclonal Antibody (Catalog # MAB610R). The ND50 is typically 0.01-0.04 μg/mL in the presence of the metabolic inhibitor actinomycin D (1 μg/mL).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
TNF-alpha, designated TNFSF2, is a trimeric glycoprotein active in both
membrane bound and secreted forms. TNF-alpha is produced by several
lymphoid cells as well as by astrocytes, endothelial cells, and smooth
muscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtually
all cell types where it triggers the activation of multiple signal
transduction pathways and modulates the expression of a wide variety of
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.