Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 729419
View all WTX Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human WTX
Met421-Leu573
Accession # Q5JTC6

Specificity

Detects human WTX in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human WTX Antibody

Detection of Human WTX antibody by Western Blot.

Detection of Human WTX by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for WTX at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

WTX antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

WTX in BG01V Human Embryonic Stem Cells.

WTX was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human WTX Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: U937 human histiocytic lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WTX

WTX (Wilms Tumor on the X; also FAM123B and AMER1) is a 190-200 kDa member of the FAM123 family of proteins. It is widely expressed in both adult and embryo and appears to have at least three functions: one, it binds WT1 in the nucleus and regulates cell differentiation; two, it associates with the plasma membrane and draws APC away from microtubules, thereby participating in intercellular junction integrity; and three, it binds beta -catenin and impacts WNT signaling. Human WTX is 1135 amino acids in length. It contains a PtdIns-P2 binding site (aa 2-209), an acidic domain (aa 370-411), and a Pro-rich region (aa 952-1104). There are three APC binding motifs (aa 280-368; 380-431; 717-834) and the beta -catenin binding region lies C-terminal to Gly368. There are two isoform variants. One is 150 kDa in size, nuclear in location, and shows a deletion of aa 50-326. A second shows a 19 aa substitution for aa 786-1135. Over aa 421-573, human WTX shares 89% aa identity with mouse WTX.

Long Name

Wilms Tumor on the X Chromosome

Alternate Names

AMER1, FAM123B, OSCS

Entrez Gene IDs

139285 (Human); 72345 (Mouse); 501584 (Rat)

Gene Symbol

AMER1

UniProt

Q5JTC6 (Human)

Additional WTX Products

Product Documents for Human WTX Antibody

Certificate of Analysis

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Product Specific Notices for Human WTX Antibody

For research use only

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Protocols

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