Human WTX Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human WTX in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 729419
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human WTX
    Met421-Leu573
    Accession # Q5JTC6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human WTX by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for WTX at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
WTX in BG01V Human Embryonic Stem Cells. WTX was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WTX
WTX (Wilms Tumor on the X; also FAM123B and AMER1) is a 190-200 kDa member of the FAM123 family of proteins. It is widely expressed in both adult and embryo and appears to have at least three functions: one, it binds WT1 in the nucleus and regulates cell differentiation; two, it associates with the plasma membrane and draws APC away from microtubules, thereby participating in intercellular junction integrity; and three, it binds beta -catenin and impacts WNT signaling. Human WTX is 1135 amino acids in length. It contains a PtdIns-P2 binding site (aa 2-209), an acidic domain (aa 370-411), and a Pro-rich region (aa 952-1104). There are three APC binding motifs (aa 280-368; 380-431; 717-834) and the beta -catenin binding region lies C-terminal to Gly368. There are two isoform variants. One is 150 kDa in size, nuclear in location, and shows a deletion of aa 50-326. A second shows a 19 aa substitution for aa 786-1135. Over aa 421-573, human WTX shares 89% aa identity with mouse WTX.
  • Long Name:
    Wilms Tumor on the X Chromosome
  • Entrez Gene IDs:
    139285 (Human); 72345 (Mouse); 501584 (Rat)
  • Alternate Names:
    AMER1; FAM123B; OSCS; WTX
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