Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 117305
View all XIAP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170

Specificity

Detects human XIAP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human XIAP Antibody

Human XIAP Antibody in ELISA Standard Curve.

Human XIAP ELISA Standard Curve.

Recombinant Human XIAP protein was serially diluted 2-fold and captured by Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8222) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human XIAP Monoclonal Antibody(Catalog # MAB8223) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human XIAP Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human XIAP Monoclonal Antibody(Catalog # MAB8223).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Total XIAP DuoSet IC ELISA Kit (Catalog # DYC822-2) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits Caspase-3 and Caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Long Name

X-linked Inhibitor of Apoptosis

Alternate Names

BIRC4

Entrez Gene IDs

331 (Human); 11798 (Mouse); 63879 (Rat)

Gene Symbol

XIAP

UniProt

P98170

Additional XIAP Products

Product Documents for Human XIAP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human XIAP Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

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