Human ZEB2/SIP1 Antibody

Catalog # Availability Size / Price Qty
MAB73782
MAB73782-SP
Detection  of ZEB2/SIP1 in A549 Human Cell Line by Flow Cytometry.
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Human ZEB2/SIP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ZEB2/SIP1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 923328
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ZEB2/SIP1
Asn363-Lys537
Accession # O60315
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection  of ZEB2/SIP1 in A549 Human Cell Line by Flow Cytometry. View Larger

Detection of ZEB2/SIP1 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human ZEB2/SIP1 Monoclonal Antibody (Catalog # MAB73782, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Immunohistochemistry ZEB2/SIP1 in Human Liver. View Larger

ZEB2/SIP1 in Human Liver. ZEB2/SIP1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human ZEB2/SIP1 Monoclonal Antibody (Catalog # MAB73782) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZEB2/SIP1

SIP1 (Smad-interacting protein 1), also called ZEB2 (Zinc finger E-box-binding homeobox 2), is a nuclear transcription factor. SIP1 contains seven C2H2-type zinc finger domains within amino acids (aa) 211-334 and 999-1076, a Smad-MHZ binding domain (aa 437-487), an atypical homeobox domain (aa 644-703), and phosphorylation, sumoylation and acetylation sites. The 1214 aa SIP1 gives a calculated molecular weight of 136 kDa, but may actually appear closer to 200 kDa due to modifications. A 1190 aa isoform lacks aa 111-134. Within aa 363-537, human SIP1 shares 98% and 97% aa sequence identity with mouse and rat SIP1, respectively. SIP1 is highly expressed in postmitotic neocortical cells and influences cell fate decisions in embryonic brain development. Point mutations causing underexpression of SIP1 are associated with Mowat-Wilson syndrome (MWIS), also known as Hirschprung disease mental retardation syndrome.

Entrez Gene IDs
9839 (Human); 24136 (Mouse); 311071 (Rat)
Alternate Names
HSPC082; KIAA0569FLJ42816; SIP1; SIP1HSPC082; Smad-interacting protein 1; SMADIP1; SMADIP1ZFHX1BSIP-1; ZEB2; ZFHX1B; ZFX1B; zinc finger E-box binding homeobox 2; zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; Zinc finger homeobox protein 1b

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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