Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
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Proteome Profiler Rat Cytokine Array Kit, Panel A
R&D Systems | Catalog # ARY008
Contains 4 membranes - each spotted in duplicate with 29 different cytokine antibodies
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Key Product Details
Species
Rat
Product Summary for Proteome Profiler Rat Cytokine Array Kit, Panel A
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.
Troubleshooting GuideKey Benefits
- Detects 29 rat cytokines and chemokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Rat Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
Kit Contents
- Rectangular 4-Well Multi-dish
- 4 Rat Cytokine Array, Panel A nitrocellulose membranes spotted with 29 different antibodies to rat cytokines and chemokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Rat Cytokine Array, Panel A
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
|---|---|---|
| CXCL1/CINC-1 | IL-1ra/IL-1F3 | L-Selectin |
| CXCL3/CINC-2 alpha/beta | IL-2 | CXCL9/MIG |
| CXCL2/CINC-3 | IL-3 | CCL3/MIP-1 alpha |
| CNTF | IL-4 | CCL20/MIP-3 alpha |
| Fractalkine/CXC3CL1 | IIL-6 | CCL5/RANTES |
| GM-CSF | IL-10 | CXCL7/Thymus Chemokine |
| ICAM-1 | IL-13 | TIMP-1 |
| IFN-gamma | IL-17 | TNF-alpha |
| IL-1 alpha/IL-1F1 | CXCL10/IP-10 | VEGF |
| IL-1 beta/IL-1F2 | LIX | |
Assays for Analytes represented in the Rat Cytokine Array Kit, Panel A
| Analyte | Quantikine® ELISA Kits | DuoSet® ELISA Development Systems |
|---|---|---|
| CXCL1/CINC-1 | DY515 | |
| CXCL3/CINC-2 alpha/beta | RCN200 | DY516 |
| CXCL2/CINC-3 | RCN300 | DY525 |
| CNTF | DY557 | |
| Fractalkine/CX3CL1 | MCX310 | DY537 |
| GM-CSF | DY518 | |
| ICAM-1 | RIC100 | DY583 |
| IFN-gamma | RIF00 | DY585 |
| IL-1 alpha | RRA00 | DY500 |
| IL-1 beta | RLB00 | DY501 |
| IL-1ra | ||
| IL-2 | R2000 | DY502 |
| IL-3 | ||
| IL-4 | R4000 | DY504 |
| IL-6 | R6000B | DY506 |
| IL-10 | R1000 | DY522 |
| IL-13 | ||
| IL-17 | ||
| CXCL10/IP-10 | ||
| LIX | DY543 | |
| L-Selectin | DY1534 | |
| CXCL9/MIG | ||
| CCL3/MIP-1 alpha | ||
| CCL20/MIP-3 alpha | DY540 | |
| CCL5/RANTES | MMR00 | |
| CXCL7/Thymus Chemokine | DY1116 | |
| TIMP-1 | RTM100 | DY580 |
| TNF-alpha | RTA00 | DY510 |
| VEGF | RRV00 | DY564 |
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Scientific Data Images for Proteome Profiler Rat Cytokine Array Kit, Panel A
The Rat Cytokine Array detects multiple analytes in cell culture supernates.
NR8383 rat alveolar macrophage cells were either untreated or treated as below. Data shown are from a two minute exposure to X-ray film. A, untreated. B, Treated with 100 ng/mL LPS for 24 hours. C, Pre-treated with 100 nM Dexamethasone for 72 hours followed by treatment with 100 ng/mL LPS for 24 hours.
The Rat Cytokine Array detects multiple analytes in tissue lysates and serum.
A rat was injected with 0.1 mg/kg LPS. After 24 hours, tissues were excised and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels A-B). Tissues were excised from untreated rats and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels C-E). Serum samples from 15 week old male rats were prepared as described in the Sample Collection and Storage section. 100 µL of serum was run on the array. Data shown are from a 5 minute exposure to X-ray film (Panel F).
The Rat Cytokine Array detects multiple analytes in cell culture supernates and lysates.
(A. and B.) Supernates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 500 μL of supernate was used for each array shown. (C. and D.) Lysates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 200 μg of lysates was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
The Rat Cytokine Array detects cytokines present in serum and tissue.
Representative membranes and selective quantification are shown for rat serum (A., 150 μL), lysates of rat lung tissue (B., 400 μg), and lysates of rat liver tissue (C., 400 μg). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Multiplex Cytokine Assay Kits
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Proteome Profiler Rat Cytokine Array Kit, Panel A
Product Specific Notices for Proteome Profiler Rat Cytokine Array Kit, Panel A
For research use only
Citations for Proteome Profiler Rat Cytokine Array Kit, Panel A
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Customer Reviews for Proteome Profiler Rat Cytokine Array Kit, Panel A (8)
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8 Customer Ratings
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Verified Customer | Posted 03/11/2021
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Verified Customer | Posted 11/15/2020
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Verified Customer | Posted 05/15/2020This kit is easy to use and would definitely use again.
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Verified Customer | Posted 02/01/2020Performed well, great tool to screen for possible targets before making a more quantitative and more expensive assay. Spots can be easily evaluated using e.g., Bio-Rad ImageLab, and the semi-quantitative analysis can basically detect substantial differences between experimental groups.
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Verified Customer | Posted 04/01/2018
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Verified Customer | Posted 01/20/2017This array kit is a very nice tool to screen potential cytokines in spent media of cell culture.
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Verified Customer | Posted 01/03/2017This kit is an excellent tool in screening potential cytokines and chemokines. However, it would be nice if more targets can be included for this Rat cytokine panel (since the mouse and human panel has XL format).
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Verified Customer | Posted 10/06/2016
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FAQs for Proteome Profiler Rat Cytokine Array Kit, Panel A
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Q: Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
A: The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.