Mouse 4-1BB/TNFRSF9/CD137 Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat 4-1BB/TNFRSF9 is observed. In Western blots, less than 5% cross-reactivity with recombinant human 4-1BB and recombinant mouse EDAR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse 4‑1BB/TNFRSF9/CD137
    Val24-Leu187
    Accession # P20334
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    1-15 µg/mL
    See below
  • Agonist Activity
    Measured by its ability to co-stimulate IFN-gamma secretion by mouse splenic T cells in the presence of anti-CD3. Saoulli, K. et al. (1998) J. Exp. Med. 187(11):1849 and Cannons, J. et al. (2001) J. Immunol. 167:1313.
    The ED50 for this effect is typically 0.3 - 1 μg/mL.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse 4‑1BB/TNFRSF9/
CD137 by Western Blot.
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with recombinant mouse 4-1BB/Fc chimera. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse
4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for
4‑1BB/TNFRSF9/CD137 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of 4‑1BB/TNFRSF9/CD137 in Activated Mouse Splenocytes by Flow Cytometry. Activated mouse splenocytes were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Goat Anti-Mouse
4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control
(Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Detection of 4‑1BB/TNFRSF9/CD137 in Resting Mouse Splenocytes by Flow Cytometry. Resting mouse splenocytes were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Goat Anti-Mouse
4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control
(Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
4‑1BB/TNFRSF9/CD137 in Mouse Spleen. 4‑1BB/TNFRSF9/CD137 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse
4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 4-1BB/TNFRSF9/CD137

4-1BB, also known as CD137 and ILA (induced by lymphocyte activation), is a TNF receptor superfamily member and has been designated TNFRSF9. Mouse 4‑1BB cDNA encodes a 256 amino acid (aa) residues type I transmembrane protein with a putative 23 aa signal peptide, a 164 aa extracellular domain, a 21 aa transmembrane domain and a 48 aa cytoplasmic region (1‑3). A soluble 4‑1BB is released from surfaces of cells expressing the transmembrane protein (4).
Mouse 4‑1BB shares approximately 60% aa sequence identity with its human counterpart. 4‑1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils (5). The ligand for 4‑1BB (4‑1BBL), also named TNFSF9, belongs to the TNF ligand superfamily. 4‑1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. In response to 4‑1BBL binding, 4‑1BB transduce a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In vivo, the costimulatory activity of 4‑1BB has been shown to be important in graft-vs-host disease and antiviral CTL responses. On dendritic cells, 4‑1BB is a DC-activating molecules that enhances cytokine production and up‑regulates expression of B7-1 and B7-2 costimulatory molecules, resulting in an improved ability to stimulate T cell responses (1‑5).

  • References:
    1. Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631.
    2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
    3. Kwon, B.S. and S.M. Weissman (1989) Proc. Nat. Acad. Sci. USA 86:1963.
    4. Wilcox, R.A. et al. (2002) J. Immunol. 168:4262.
    5. Kwon, B., H.W. Lee and B.S. Kwon, 2002, TRENDS in Immunology 23:378.
  • Entrez Gene IDs:
    3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)
  • Alternate Names:
    4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Isoproterenol Increases Uncoupling, Glycolysis, and Markers of Beiging in Mature 3T3-L1 Adipocytes.
    Authors: Miller C, Yang J, England E, Yin A, Baile C, Rayalam S
    PLoS ONE, 2015;10(9):e0138344.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Fresh
  2. Agonist anti-CD137 mAb act on tumor endothelial cells to enhance recruitment of activated T lymphocytes.
    Authors: Palazon A, Teijeira A, Martinez-Forero I, Hervas-Stubbs S, Roncal C, Penuelas I, Dubrot J, Morales-Kastresana A, Perez-Gracia JL, Ochoa MC, Ochoa-Callejero L, Martinez A, Luque A, Dinchuk J, Rouzaut A, Jure-Kunkel M, Melero I
    Cancer Res., 2011;71(3):801-11.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
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