Mouse CD25/IL-2R alpha Antibody

Cell Proliferation Induced by IL‑2 and Neutralization by Mouse CD25IL‑2 R alpha Antibody.

Recombinant Mouse IL-2 (402-ML) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438). The ND₅₀ is typically 5-20 µg/mL.

CD25/IL‑2 R alpha in Mouse Spleen.

CD25/IL-2 Ra was detected in immersion fixed frozen sections of mouse spleen using 5 µg/mL Goat Anti-Mouse CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Mouse CD25/IL-2R alpha by Flow Cytometry

The persistent presence of fungi in lung injury induced by Aspergillus fumigatus is related to Tregs. (a) Increased CD4+CD25+Foxp3+ Treg proportion significant in PBMCs of patients infected with A. fumigatus (n = 7). (b) Foxp3 mRNA levels in the lungs were measured with q-PCR. Relative expression levels of the genes were expressed with the GAPDH housekeeping gene as an internal reference (n = 5/group). The expression of Foxp3 protein levels in the lungs was measured with Western blotting. Relative expression levels were expressed with the GAPDH as an internal reference (n = 3/group). (c) CD4+CD25+ T cells and CD4+CD25+Foxp3+ Tregs in the spleen were detected by flowcytometry, and FlowJo10 analyzed the proportions as prior described in Section 2. Increased CD4+CD25+ T cell and CD4+CD25+Foxp3+ Treg proportion significantly in the spleen of A. fumigatus infection mice (n = 5/group).Decreased susceptibility of mice to A. fumigatus infection after Treg depletion. (d) After pretreatment with CD25-neutralizing antibodies, the number of Treg cells in the spleen of mice with A. fumigatus infection group was significantly reduced, each group (n = 5/group). (e) Fungal load (colony-forming unit) after 1 day of infection (n = 4/group). Experiments were done at least three times. Data are presented as mean ± standard deviation. ∗Statistically significant difference (p < 0.05) against noninfected control. (f) Lungs from each experimental group were processed for histological examination after H&E staining. Lung injury scores were evaluated by the method described previously. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 by Student's unpaired two-tailed t test and the one-way ANOVA followed by the Tukey post hoc test. Error bars represent SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34222495), licensed under a CC-BY license. Not internally tested by R&D Systems.