Mouse HGF Antibody

(11 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse HGF in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) HGF, rhHGF R, recombinant mouse (rm) HGF R, rhHGF-A, and rmHGF-A is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse HGF (R&D Systems, Catalog # 2207-HG)
    Gln33-Leu728
    Accession # Q53WS5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse HGF (Catalog # 2207-HG)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse HGF Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse HGF Antibody (Catalog # AF2207)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse HGF Biotinylated Antibody (Catalog # BAF2207)
  • ELISA Standard
     
    Recombinant Mouse HGF Protein (Catalog # 2207-HG)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
HGF in Mouse Liver. HGF was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
HGF in Mouse Skeletal Muscle. HGF was detected in perfusion fixed frozen sections of mouse skeletal muscle using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF

HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91%‑95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

  • References:
    1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
    2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
    3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
    4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
    5. Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
    6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
    7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
    8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
    9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
    10. Montesano, R. et al. (1991) Cell 67:901.
    11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
    12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
    13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
    14. Mineo, R. et al. (1994) Endocrinology 145:4355.
    15. Urbanek, K. et al. (2005) Circ. Res. 97:663.
  • Long Name:
    Hepatocyte Growth Factor
  • Entrez Gene IDs:
    3082 (Human); 15234 (Mouse); 403441 (Canine)
  • Alternate Names:
    deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Progesterone dose-dependently modulates hepatocyte growth factor production in 3T3-L1 mouse preadipocytes
    Authors: T Ito, D Yamaji, A Kamikawa, MA Abd Eldaim, Y Okamatsu-O, A Terao, M Saito, K Kimura
    Endocr. J., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Epigenetic regulation of HGF/Met receptor axis is critical for the outgrowth of bone metastasis from breast carcinoma
    Authors: P Bendinelli, P Maroni, E Matteucci, MA Desiderio
    Cell Death Dis, 2017;8(2):e2578.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma
    PLoS ONE, 2016;11(5):e0156151.
    Species: Mouse
    Sample Type: N/A
    Application: Not Specified
  4. MET is required for the recruitment of anti-tumoural neutrophils.
    Authors: Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M
    Nature, 2015;522(7556):349-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
    Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
    Cell Death Differ, 2015;22(7):1219-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  6. Bone marrow progenitor cell therapy-mediated paracrine regulation of cardiac miRNA-155 modulates fibrotic response in diabetic hearts.
    Authors: Kishore, Raj, Verma, Suresh K, Mackie, Alexande, Vaughan, Erin E, Abramova, Tatiana, Aiko, Ito, Krishnamurthy, Prasanna
    PLoS ONE, 2013;8(4):e60161.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  7. Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis.
    Authors: Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H
    Cancer Res, 2013;73(8):2659-70.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  8. Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling.
    Authors: Tesio M, Golan K, Corso S
    Blood, 2011;117(2):419-28.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  9. c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74.
    Authors: Gordin M, Tesio M, Cohen S
    J. Immunol., 2010;185(4):2020-31.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
    Authors: Narasaraju T, Ng HH, Phoon MC
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
    Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
    Am. J. Pathol., 2006;169(5):1767-83.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
Expand to show all 11 Citations
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunoflourescence.

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  Very Good
 Immunocytochemistry/Immunoflourescence Mouse Anonymous 12/04/2015
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Summary

ApplicationImmunocytochemistry/Immunoflourescence
Sample TestedCultured cells and tissues
SpeciesMouse

Other Experimental Details

Other Experimental DetailsWe tested this antibody, mHGF on our cultured cells and skin tissue with immunofluorescent and immunohistochemical staining techniques. The results are very satisfactory. This mHGF antibody binds specifically to the epitope on cultured hematopoietic stem cells that we isolated from adult FVB mice. We like this antibody for its sensitivity and specificity. The provided small package provided a very good pattern of flexibility for users too.

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