Mouse HGF Antibody

Catalog # Availability Size / Price Qty
AF2207
AF2207-SP

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HGF in Mouse Liver.
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Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (2)

Mouse HGF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HGF in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HGF (R&D Systems, Catalog # 2207-HG)
Gln33-Leu728
Accession # Q53WS5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse HGF (Catalog # 2207-HG)
Immunohistochemistry
5-15 µg/mL
See below

Mouse HGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse HGF Biotinylated Antibody (Catalog # BAF2207)

Standard: Recombinant Mouse HGF Protein (Catalog # 2207-HG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry HGF antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

HGF in Mouse Liver. HGF was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry HGF antibody in Mouse Skeletal Muscle by Immunohistochemistry (IHC-Fr). View Larger

HGF in Mouse Skeletal Muscle. HGF was detected in perfusion fixed frozen sections of mouse skeletal muscle using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF

HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91%‑95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

References
  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.
Long Name
Hepatocyte Growth Factor
Entrez Gene IDs
3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 493705 (Feline)
Alternate Names
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

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Citations for Mouse HGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Epigenetic regulation of HGF/Met receptor axis is critical for the outgrowth of bone metastasis from breast carcinoma
    Authors: P Bendinelli, P Maroni, E Matteucci, MA Desiderio
    Cell Death Dis, 2017;8(2):e2578.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Progesterone dose-dependently modulates hepatocyte growth factor production in 3T3-L1 mouse preadipocytes
    Authors: T Ito, D Yamaji, A Kamikawa, MA Abd Eldaim, Y Okamatsu-O, A Terao, M Saito, K Kimura
    Endocr. J., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma
    PLoS ONE, 2016;11(5):e0156151.
    Species: Mouse
    Sample Types: N/A
    Applications: Not Specified
  4. MET is required for the recruitment of anti-tumoural neutrophils.
    Authors: Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M
    Nature, 2015;522(7556):349-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
    Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
    Cell Death Differ, 2015;22(7):1219-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  6. Bone marrow progenitor cell therapy-mediated paracrine regulation of cardiac miRNA-155 modulates fibrotic response in diabetic hearts.
    Authors: Kishore, Raj, Verma, Suresh K, Mackie, Alexande, Vaughan, Erin E, Abramova, Tatiana, Aiko, Ito, Krishnamurthy, Prasanna
    PLoS ONE, 2013;8(4):e60161.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis.
    Authors: Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H
    Cancer Res, 2013;73(8):2659-70.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling.
    Authors: Tesio M, Golan K, Corso S
    Blood, 2011;117(2):419-28.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  9. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
    Authors: Narasaraju T, Ng HH, Phoon MC
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74.
    Authors: Gordin M, Tesio M, Cohen S
    J. Immunol., 2010;185(4):2020-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
    Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
    Am. J. Pathol., 2006;169(5):1767-83.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Mouse HGF Antibody
By Anonymous on 10/13/2021
Application: IHC Sample Tested: Breast cancer tissue Species: Mouse

Mouse HGF Antibody
By Min Hu on 12/04/2015
Application: Immunocytochemistry/Immunoflourescence Sample Tested: Cultured cells and tissues Species: Mouse

We tested this antibody, mHGF on our cultured cells and skin tissue with immunofluorescent and immunohistochemical staining techniques. The results are very satisfactory. This mHGF antibody binds specifically to the epitope on cultured hematopoietic stem cells that we isolated from adult FVB mice.

We like this antibody for its sensitivity and specificity. The provided small package provided a very good pattern of flexibility for users too.