Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HGF Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse HGF (R&D Systems, Catalog # 2207-HG)
Gln33-Leu728
Accession # Q53WS5

Specificity

Detects mouse HGF in ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse HGF Antibody

HGF antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

HGF in Mouse Liver.

HGF was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

HGF antibody in Mouse Skeletal Muscle by Immunohistochemistry (IHC-Fr).

HGF in Mouse Skeletal Muscle.

HGF was detected in perfusion fixed frozen sections of mouse skeletal muscle using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse HGF Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse skeletal muscle and mouse liver

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse HGF (Catalog # 2207-HG)

Mouse HGF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse HGF Biotinylated Antibody (Catalog # BAF2207)
  • Standard: Recombinant Mouse HGF Protein (Catalog # 2207-HG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.5 using AF2207 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: HGF

HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91%‑95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

References

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Long Name

Hepatocyte Growth Factor

Alternate Names

DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF

Entrez Gene IDs

3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)

Gene Symbol

HGF

UniProt

Q53WS5

Additional HGF Products

Product Documents for Mouse HGF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse HGF Antibody

For research use only

Citations for Mouse HGF Antibody

Customer Reviews for Mouse HGF Antibody (4)

4.5 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse HGF Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Mouse HGF Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Mouse
    Verified Customer | Posted 10/13/2021
    Mouse HGF Antibody AF2207
  • Mouse HGF Antibody
    Name: Min Hu
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Cultured cells and tissues
    Species: Mouse
    Verified Customer | Posted 12/04/2015
    We tested this antibody, mHGF on our cultured cells and skin tissue with immunofluorescent and immunohistochemical staining techniques. The results are very satisfactory. This mHGF antibody binds specifically to the epitope on cultured hematopoietic stem cells that we isolated from adult FVB mice. We like this antibody for its sensitivity and specificity. The provided small package provided a very good pattern of flexibility for users too.
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 23074208
    Species: Mouse
    Verified Customer | Posted 01/06/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 24218051
    Species: Mouse
    Verified Customer | Posted 01/06/2015

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies