|Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (10 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA). The ND50 is typically 2-12 µg/mL in the presence of concanavalin A (1.25 µg/mL).|
Detection of Human and Mouse|
IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 4 hours and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours. PVDF membrane was probed with µg/mL of Goat Anti-Mouse
IL‑1 beta /IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA). This experiment was conducted under reducing conditions and using the|
12-230 kDa separation system.
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta.
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