Mouse Neprilysin/CD10 Antibody

Catalog # Availability Size / Price Qty
AF1126
AF1126-SP
Neprilysin/CD10 in Mouse Brain.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)

Mouse Neprilysin/CD10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Neprilysin/CD10 in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Neprilysin/CD10
Tyr52-Trp750
Accession # AAA37386
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Neprilysin/CD10 (Catalog # 1126-ZN)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Neprilysin/CD10 (Catalog # 1126-ZN), see our available Western blot detection antibodies

Mouse Neprilysin/CD10 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1126)

Standard: Recombinant Mouse Neprilysin Protein, CF (Catalog # 1126-ZN)

Neutralization
Measured by its ability to neutralize Recombinant Mouse Neprilysin/CD10 (0.1 μg/mL, Catalog # 1126-ZN) cleavage of the fluorogenic peptide substrate Mca-RPPGFSAFK(Dnp)-OH (10 µM, Catalog # ES005). The Neutralization Dose (ND50) is typically 1.3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Neprilysin/CD10 in Mouse Brain. View Larger

Neprilysin/CD10 in Mouse Brain.

Neprilysin/CD10 was detected in perfusion fixed frozen sections of mouse brain (glial cell in hippocampus) using 15 µg/mL Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neprilysin/CD10

Neprilysin (NEP, neutral endopeptidase 24.11, EC 3.4.24.11) is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphoblastic leukemia antigen (CALLA), and to be expressed on the surface of lymphocytes in some disease states (3). These and other observations have resulted in considerable clinical interest in NEP as a potential target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (4).

References
  1. Malfroy, B. et al. (1978) Nature 276:523.
  2. Kenny, A.J. and S.L. Stephenson (1988) FEBS Lett. 232:1.
  3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
  4. Itwata, N. et al. (2001) Science 292:1550.
Entrez Gene IDs
4311 (Human); 17380 (Mouse); 24590 (Rat)
Alternate Names
Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase

Product Datasheets

Citations for Mouse Neprilysin/CD10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice
    Authors: K Kidana, T Tatebe, K Ito, N Hara, A Kakita, T Saito, S Takatori, Y Ouchi, T Ikeuchi, M Makino, TC Saido, M Akishita, T Iwatsubo, Y Hori, T Tomita
    EMBO Mol Med, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC - Frozen
  2. Pericyte loss influences Alzheimer-like neurodegeneration in mice.
    Authors: Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B
    Nat Commun, 2013;4(0):2932.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  3. Expression of neprilysin in skeletal muscle reduces amyloid burden in a transgenic mouse model of Alzheimer disease.
    Authors: Liu Y, Studzinski C, Beckett T, Guan H, Hersh MA, Murphy MP, Klein R, Hersh LB
    Mol. Ther., 2009;17(8):1381-6.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse Neprilysin/CD10 Antibody

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Mouse Neprilysin/CD10 Antibody
By Anonymous on 08/26/2016
Application: WB Sample Tested: Kidney tissue Species: Rat

Mouse Neprilysin/CD10 Antibody
By Anonymous on 04/26/2016
Application: WB Sample Tested: Kidney tissue Species: Mouse