Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat, Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Neprilysin/CD10 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Neprilysin/CD10
Tyr52-Trp750
Accession # AAA37386

Specificity

Detects mouse Neprilysin/CD10 in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Neprilysin/CD10 Antibody

Neprilysin/CD10 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Neprilysin/CD10 in Mouse Brain.

Neprilysin/CD10 was detected in perfusion fixed frozen sections of mouse brain (glial cell in hippocampus) using 15 µg/mL Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse Neprilysin/CD10 by Western Blot

Detection of Mouse Neprilysin/CD10 by Western Blot

Astrocyte‐specific Stat3 deletion increases microglial A beta internalization and degradation, and reduces apoE expression, dystrophic neurites, and detrimental cytokinesAInternalization of A beta (stained with IC16 antibody or methoxy‐XO4) was assessed using an engulfment assay, in which glial and A beta structures were surface‐rendered and A beta volumes co‐localized with glial volumes were quantified. Scale bars, 10 μm.B, CMicroglia (left Y axes) from APP/PS1 mice internalized significantly more A beta positive for IC16 or methoxy‐XO4 when Stat3 was deleted in astrocytes (*P < 0.05, Mann–Whitney test), whereas no changes were seen in astrocytes (right axes; APP/PS1‐Stat3WT, n = 8 (four females and four males) mice; APP/PS1‐Stat3KO, n = 11 (five females and six males) mice; age, 11 months; Mann–Whitney test).D–H(D–F) Western blot quantification of protein levels of the A beta ‐degrading enzymes neprilysin/CD10 and CD36, as well as the A beta ‐binding apolipoprotein E (apoE), revealed a significantly increased expression of neprilysin and CD36 and a decreased expression of apoE (APP/PS1‐Stat3WT, n = 9 (five females and four males) mice; APP/PS1‐Stat3KO, n = 9 (five females and four males) mice; age, 11 months; *P < 0.05, Mann–Whitney test for all comparisons). (G) In contrast, TREM2 expression remained unchanged (APP/PS1‐Stat3WT, n = 8 (four females and four males) mice; APP/PS1‐Stat3KO, n = 7 (four females and three males) mice; age, 11 months; Mann–Whitney test). (H) Western blots for proteins analyzed in (D‐G).Data information: Data are represented as mean ± SEM.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30617153), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Neprilysin/CD10 by Western Blot

Detection of Mouse Neprilysin/CD10 by Western Blot

Astrocyte‐specific Stat3 deletion increases microglial A beta internalization and degradation, and reduces apoE expression, dystrophic neurites, and detrimental cytokinesAInternalization of A beta (stained with IC16 antibody or methoxy‐XO4) was assessed using an engulfment assay, in which glial and A beta structures were surface‐rendered and A beta volumes co‐localized with glial volumes were quantified. Scale bars, 10 μm.B, CMicroglia (left Y axes) from APP/PS1 mice internalized significantly more A beta positive for IC16 or methoxy‐XO4 when Stat3 was deleted in astrocytes (*P < 0.05, Mann–Whitney test), whereas no changes were seen in astrocytes (right axes; APP/PS1‐Stat3WT, n = 8 (four females and four males) mice; APP/PS1‐Stat3KO, n = 11 (five females and six males) mice; age, 11 months; Mann–Whitney test).D–H(D–F) Western blot quantification of protein levels of the A beta ‐degrading enzymes neprilysin/CD10 and CD36, as well as the A beta ‐binding apolipoprotein E (apoE), revealed a significantly increased expression of neprilysin and CD36 and a decreased expression of apoE (APP/PS1‐Stat3WT, n = 9 (five females and four males) mice; APP/PS1‐Stat3KO, n = 9 (five females and four males) mice; age, 11 months; *P < 0.05, Mann–Whitney test for all comparisons). (G) In contrast, TREM2 expression remained unchanged (APP/PS1‐Stat3WT, n = 8 (four females and four males) mice; APP/PS1‐Stat3KO, n = 7 (four females and three males) mice; age, 11 months; Mann–Whitney test). (H) Western blots for proteins analyzed in (D‐G).Data information: Data are represented as mean ± SEM.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30617153), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immersion fixed paraffin-embedded sections of mouse kidney

Neprilysin/CD10 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immersion fixed paraffin-embedded sections of mouse spinal cord

Neprilysin/CD10 was detected in immersion fixed paraffin-embedded sections of mouse spinal cord using Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Mouse Neprilysin/CD10 Antibody

Application
Recommended Usage

Immunohistochemistry

0.25-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (glial cell in hippocampus), Immersion fixed paraffin-embedded sections of mouse spinal cord, and of mouse kidney

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Neprilysin/CD10 (Catalog # 1126-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Neprilysin/CD10 (Catalog # 1126-ZN)

Mouse Neprilysin/CD10 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1126)
  • Standard: Recombinant Mouse Neprilysin Protein, CF (Catalog # 1126-ZN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using AF1126 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neprilysin/CD10

Neprilysin (NEP, neutral endopeptidase 24.11, EC 3.4.24.11) is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphoblastic leukemia antigen (CALLA), and to be expressed on the surface of lymphocytes in some disease states (3). These and other observations have resulted in considerable clinical interest in NEP as a potential target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (4).

References

  1. Malfroy, B. et al. (1978) Nature 276:523.
  2. Kenny, A.J. and S.L. Stephenson (1988) FEBS Lett. 232:1.
  3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
  4. Itwata, N. et al. (2001) Science 292:1550.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Entrez Gene IDs

4311 (Human); 17380 (Mouse); 24590 (Rat); 101925772 (Cynomolgus Monkey)

Gene Symbol

MME

UniProt

AAA37386

Additional Neprilysin/CD10 Products

Product Documents for Mouse Neprilysin/CD10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Neprilysin/CD10 Antibody

For research use only

Citations for Mouse Neprilysin/CD10 Antibody

Customer Reviews for Mouse Neprilysin/CD10 Antibody (2)

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  • Mouse Neprilysin/CD10 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Kidney tissue
    Species: Rat
    Verified Customer | Posted 08/26/2016
    Mouse Neprilysin/CD10 Antibody AF1126
  • Mouse Neprilysin/CD10 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 04/26/2016
    Mouse Neprilysin/CD10 Antibody AF1126

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