Mouse Neprilysin/CD10 Antibody

• Species Reactivity
  Mouse
• Specificity
  Detects mouse Neprilysin/CD10 in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Neprilysin/CD10
  Tyr52-Trp750
  Accession # AAA37386
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.1 µg/mL
  Recombinant Mouse Neprilysin/CD10 (Catalog # 1126-ZN)
• Immunohistochemistry
  5-15 µg/mL
  See below
• Immunoprecipitation
  25 µg/mL
  Conditioned cell culture medium spiked with Recombinant Mouse Neprilysin/CD10 (Catalog # 1126-ZN), see our available Western blot detection antibodies
• Mouse Neprilysin/CD10 Sandwich Immunoassay
  Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 
Mouse Neprilysin/CD10 Antibody (Catalog # AF1126 )
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 
Mouse Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1126 )
ELISA Standard
 
Recombinant Mouse Neprilysin Protein, CF (Catalog # 1126-ZN )
Neutralization
Measured by its ability to neutralize Recombinant Mouse Neprilysin/CD10 (0.1 μg/mL, Catalog # 1126-ZN) cleavage of the fluorogenic peptide substrate Mca-RPPGFSAFK(Dnp)-OH (10 µM, Catalog # ES005). The Neutralization Dose (ND₅₀) is typically 1.3 µg/mL.

Immunohistochemistry click image to view larger

Neprilysin/CD10 in Mouse Brain. Neprilysin/CD10 was detected in perfusion fixed frozen sections of mouse brain (glial cell in hippocampus) using 15 µg/mL Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Malfroy, B. et al. (1978) Nature 276:523.
  2. Kenny, A.J. and S.L. Stephenson (1988) FEBS Lett. 232:1.
  3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
  4. Itwata, N. et al. (2001) Science 292:1550.

• Entrez Gene IDs:
  4311 (Human); 17380 (Mouse); 24590 (Rat)

• Alternate Names:
  Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase

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