Mouse Neprilysin/CD10 Antibody Summary
Accession # AAA37386
Mouse Neprilysin/CD10 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Neprilysin/CD10 in Mouse Brain.
Neprilysin/CD10 was detected in perfusion fixed frozen sections of mouse brain (glial cell in hippocampus) using 15 µg/mL Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Neprilysin (NEP, neutral endopeptidase 24.11, EC 188.8.131.52) is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphoblastic leukemia antigen (CALLA), and to be expressed on the surface of lymphocytes in some disease states (3). These and other observations have resulted in considerable clinical interest in NEP as a potential target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (4).
- Malfroy, B. et al. (1978) Nature 276:523.
- Kenny, A.J. and S.L. Stephenson (1988) FEBS Lett. 232:1.
- LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
- Itwata, N. et al. (2001) Science 292:1550.
Citations for Mouse Neprilysin/CD10 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice
Authors: K Kidana, T Tatebe, K Ito, N Hara, A Kakita, T Saito, S Takatori, Y Ouchi, T Ikeuchi, M Makino, TC Saido, M Akishita, T Iwatsubo, Y Hori, T Tomita
EMBO Mol Med, 2018;0(0):.
Sample Types: Whole Cells
Applications: IHC - Frozen
Pericyte loss influences Alzheimer-like neurodegeneration in mice.
Authors: Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B
Nat Commun, 2013;4(0):2932.
Sample Types: Tissue Homogenates
Expression of neprilysin in skeletal muscle reduces amyloid burden in a transgenic mouse model of Alzheimer disease.
Authors: Liu Y, Studzinski C, Beckett T, Guan H, Hersh MA, Murphy MP, Klein R, Hersh LB
Mol. Ther., 2009;17(8):1381-6.
Sample Types: Tissue Homogenates
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