Mouse/Rat FABP4/A-FABP Antibody

Catalog # Availability Size / Price Qty
AF1443
AF1443-SP
Detection of Mouse FABP4/A‑FABP by Western Blot.
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Product Details
Citations (23)
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Mouse/Rat FABP4/A-FABP Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse FABP4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) FABP5 and rmFABP9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse FABP4
Met1-Ala132
Accession # P04117
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse FABP4/A-FABP antibody by Western Blot. View Larger

Detection of Mouse FABP4/A‑FABP by Western Blot. Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry FABP4 antibody in Mouse Adipocytes by Immunocytochemistry (ICC). View Larger

FABP4 in Mouse Adipocytes. FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry FABP4 antibody in Rat Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

FABP4 in Rat Mesenchymal Stem Cells. FABP4 was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry FABP4 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

FABP4 in Mouse Intestine. FABP4 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse FABP4/A-FABP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse FABP4/A‑FABP by Simple WesternTM. Simple Western lane view shows lysates of mouse thymus tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A-FABP at approximately 17 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse/Rat FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Mouse FABP4/A-FABP by Western Blot View Larger

Detection of Mouse FABP4/A-FABP by Western Blot A-FABP mediates expression of Dio2 via inhibition of LXR alpha.(a,b) BAT isolated from WT and A-FABP KO mice (a) fed with STC or HFD for 24 weeks or (b) subjected to room temperature (23 °C) or cold exposure (6 °C) for 24 h as indicated in Fig. 2 were subjected to immunoblotting using an antibody against A-FABP, type II iodothyronine deiodinase (D2), liver X receptor alpha (LXR alpha ) and beta -tubulin. The bar charts below are the band intensity of each protein relative to beta -tubulin and expressed as arbitrary units, N.D., not detected (n=6). (c,d) The mRNA abundance of A-FABP, Dio2 and LXR alpha in BAT of above WT and A-FABP KO mice (c) fed with STC or HFD for 24 weeks or (d) subjected to cold exposure (6 °C) for 24 h (n=6). (e) The mRNA abundance of A-FABP, LXR alpha and Dio2 in WT or A-FABP-deficient primary brown adipocytes incubated with PBS or recombinant A-FABP (rA-FABP, 2 μg ml−1) for 24 h (n=6). (f) The mRNA abundance of LXR alpha downstream target genes (SCD-1, SREBP-1c) and Dio2 in WT and A-FABP-deficient primary brown adipocytes treated with or without LXR alpha agonist TO901317 (TO;1 μM) and/or rA-FABP (2 μg ml−1) for 24 h (n=6). Uncropped western blot images are shown in Supplementary Fig. 13. Data are represented as mean±s.e.m. *P<0.05, **P<0.01 (One-way analysis of variance with Bonferroni correction for multiple comparisons). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28128199), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse FABP4/A-FABP by Western Blot View Larger

Detection of Mouse FABP4/A-FABP by Western Blot A-FABP accelerates proteasomal degradation of LXR alpha.(a) Primary adipocytes derived from male 6-week-old A-FABP KO mice or WT littermates were treated with actinomycin D (actD, 1 μg ml−1) or vehicle (PBS). The mRNA level of LXR alpha was determined by real-time PCR at time points as indicated (n=4). (b,c) Primary brown adipocytes derived from male 6-week-old A-FABP KO mice or WT littermates were treated with (b) cycloheximide (CHX, 50 μg ml−1) or (c) CHX (50 μg ml−1) together with MG132 (10 μM) for different periods were subjected to immunoblotting using an antibody against LXR alpha, A-FABP and GADPH as indicated (n=4). (d) Primary brown adipocytes derived male 6-week-old A-FABP KO mice or WT littermates were infected with adenovirus overexpressing A-FABP (Ad-AFABP) or luciferase (Ad-Luci) for 48 h, followed by treatment with CHX (50 μg ml−1) for 0,6,12 and 24 h, and then subjected to immunoblotting using an antibody against LXR alpha, A-FABP and GADPH as indicated (n=4). The right panel is the band intensity of LXR alpha normalized with GAPDH, and expressed as percentage relative to baseline (0 h). Uncropped western blot images are shown in Supplementary Fig. 14. Data are represented as mean±s.e.m. *P<0.05, **P<0.01 (Students' t-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28128199), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse FABP4/A-FABP by Western Blot View Larger

Detection of Mouse FABP4/A-FABP by Western Blot NPGPx is a suppressor of adipogenesis via ROS regulationRelative NPGPx expression assayed by qRT-PCR (left panel) and Western blots (right panel) in the stromal vascular fraction (SVF) and adipocyte fraction isolated from the gonadal fat of C57BL/6 mice (n = 3 per group).Relative NPGPx expression in preadipocytes (Lin−CD34+CD29+Sca1+), endothelial cells (CD31+), macrophages (CD11b+) and adipocytes of adipose tissue measured by in C57BL/6 mice (n = 3 per group).NPGPx, CCAAT/enhancer-binding protein beta (C/EBP beta ) and peroxisome proliferator-activated receptor gamma (PPAR gamma ) mRNA level during induced 3T3-L1 adipogenesis (n = 5 per group).NPGPx expression assayed by Western blots during 3T3L1 adipogenesis.NBT reduction staining of wild-type SVF cells (WT), NPGPx-knockout SVF cells (KO) and NPGPx-knockout SVF cells expressing human NPGPx (KO + hNPGPx) (left panels). Quantification of NBT reduction stains 8 days after induction (n = 3 per group) (right panels). *p = 0.0002 for WT vs. KO by independent Student's t-test.Oil Red O stain of WT, KO and KO + hNPGPx SVF (left panels). Quantification of Oil Red O stain 8 days after induction (n = 3 per group) (right panels). *p = 0.0002 for WT vs. KO by independent Student's t-test.Western blots showing ap2 and adiponectin expression in WT, KO and KO + hNPGPx SVF cells 8 days after induction.NBT reduction stain of NPGPx knockdown (KD) and control (V) 3T3-L1 preadipocytes after adipogenic induction (left panels). Quantification of NBT reduction stain at day 8 (n = 3 per group) (right panels). *p = 0.0004 by independent Student's t-test.Oil Red O staining of KD and V 3T3-L1 preadipocytes after adipogenic induction (left panels). Quantification of Oil Red O stain at day 8 (n = 3 per group) (right panels). *p = 0.0005 by independent Student's t-test.Western blots showing ap2 and adiponectin expression in NPGPx KD and V 3T3-L1 preadipocytes after adipogenic stimulation.NBT reduction stain and Oil Red O stain of NPGPx KD and V 3T3-L1 cells 8 days after induction. Cells were treated at with NAC at various doses the first 2 days after induction.Quantification of NBT reduction stain and Oil Red O stain in (K) (n = 3 per group).Correlation (R = 0.96) between ROS levels (Rel. NBT staining) and triglyceride contents (Rel. Oil-Red-O staining) in NPGPx KD and V 3T3-L1 cells 8 days after induction.Quantification of Oil Red O staining of NPGPx KD and V 3T3-L1 cells 8 days after induction. Cells were treated with H2O2 at different concentrations the first 2 days after induction (n = 3 per group). *p = 0.006, **p = 0.006, #p < 0.0001, ##p < 0.0001 by independent Student's t-test. All values are presented as means ± S.E.M. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23828861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse FABP4/A-FABP by Western Blot View Larger

Detection of Mouse FABP4/A-FABP by Western Blot A-FABP mediates expression of Dio2 via inhibition of LXR alpha.(a,b) BAT isolated from WT and A-FABP KO mice (a) fed with STC or HFD for 24 weeks or (b) subjected to room temperature (23 °C) or cold exposure (6 °C) for 24 h as indicated in Fig. 2 were subjected to immunoblotting using an antibody against A-FABP, type II iodothyronine deiodinase (D2), liver X receptor alpha (LXR alpha ) and beta -tubulin. The bar charts below are the band intensity of each protein relative to beta -tubulin and expressed as arbitrary units, N.D., not detected (n=6). (c,d) The mRNA abundance of A-FABP, Dio2 and LXR alpha in BAT of above WT and A-FABP KO mice (c) fed with STC or HFD for 24 weeks or (d) subjected to cold exposure (6 °C) for 24 h (n=6). (e) The mRNA abundance of A-FABP, LXR alpha and Dio2 in WT or A-FABP-deficient primary brown adipocytes incubated with PBS or recombinant A-FABP (rA-FABP, 2 μg ml−1) for 24 h (n=6). (f) The mRNA abundance of LXR alpha downstream target genes (SCD-1, SREBP-1c) and Dio2 in WT and A-FABP-deficient primary brown adipocytes treated with or without LXR alpha agonist TO901317 (TO;1 μM) and/or rA-FABP (2 μg ml−1) for 24 h (n=6). Uncropped western blot images are shown in Supplementary Fig. 13. Data are represented as mean±s.e.m. *P<0.05, **P<0.01 (One-way analysis of variance with Bonferroni correction for multiple comparisons). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28128199), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse FABP4/A-FABP by Western Blot View Larger

Detection of Mouse FABP4/A-FABP by Western Blot Circulating A-FABP facilitates the uptake of free fatty acid into adipocytes.(a) Circulating A-FABP and (b) FFA profile of male 4-week-old C57BL/6N mice fed with HFD for 24 weeks (n=8). (c) Circulating A-FABP and (d) FFA level of male 8-week-old C57BL/6N mice during cold exposure (6 °C) for 4 h (n=8). (e) Circulating A-FABP and (f) FFA level of male 8-week-old C57BL/6N mice intraperitoneally injected with norepinephrine (NE; 1 mg kg−1) or PBS (vehicle) for 4 h under fasting condition (n=6). (g) Co-immunoprecipitation (Co-IP) of A-FABP and 3H-palmitate in serum of male 8-week-old C57BL/6N mice after administration of 3H-palmitate (2 μCi) for 4 h. Right panel is the 3H-palmitate radioactivity of the co-immunoprecipitated A-FABP protein (n=6). (h,i) 3H-palmitate uptake in BAT and WAT of 8-week-old A-FABP KO mice and their WT littermates infused with PBS or (h) recombinant A-FABP (rA-FABP; 1 μg h−1) or (i) mutant R126Q (1 μg h−1) (n=6). (j) BODIPY-FA uptake in WT or A-FABP-deficient brown adipocytes treated with PBS or rA-FABP (2 μg ml−1) for 10 min (min) (n=6). (k) 3H-palmitate uptake in A-FABP-deficient adipocytes incubated with PBS, bovine serum albumin (BSA; 3 μg ml−1) or rA-FABP (2 μg ml−1) (n=6). (l) In vitro fluorescent imaging analysis of brown adipocytes treated with BODIPY-FA (2 μM) with or without pre-incubation with fluorescent-labelled rA-FABP (2 μg ml−1). Images were taken at 5, 10 and 30 min after treatment. Control image was taken at 30 min in which A-FABP-deficient brown adipocytes were incubated with BODIPY-FA without pre-incubation with rA-FABP. Scale bar, 20 μm, with magnification of 400 ×. Representative images from three independent experiments are shown (n=6). (m) Oxygen consumption rate (OCR) and its mean value (lower panel) of A-FABP-deficient brown adipocytes treated with palmitate (PA: 200 nM) with or without pre-incubation with rA-FABP (2 μg ml−1) (n=6). CPMA, count per minutes for beta particles; RFU, relative fluorescence units; OCR, oxygen consumption rate; FCCP, carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone; R/A, rotenone/antimycin A. Uncropped image for co-immunoprecipitation is shown in Supplementary Fig. 13. Data are represented as mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001 (Student's t-test (a–g), one-way analysis of variance with Bonferroni correction for multiple comparisons (h–k,m)). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28128199), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse FABP4/A-FABP by Western Blot View Larger

Detection of Mouse FABP4/A-FABP by Western Blot NPGPx is a suppressor of adipogenesis via ROS regulationRelative NPGPx expression assayed by qRT-PCR (left panel) and Western blots (right panel) in the stromal vascular fraction (SVF) and adipocyte fraction isolated from the gonadal fat of C57BL/6 mice (n = 3 per group).Relative NPGPx expression in preadipocytes (Lin−CD34+CD29+Sca1+), endothelial cells (CD31+), macrophages (CD11b+) and adipocytes of adipose tissue measured by in C57BL/6 mice (n = 3 per group).NPGPx, CCAAT/enhancer-binding protein beta (C/EBP beta ) and peroxisome proliferator-activated receptor gamma (PPAR gamma ) mRNA level during induced 3T3-L1 adipogenesis (n = 5 per group).NPGPx expression assayed by Western blots during 3T3L1 adipogenesis.NBT reduction staining of wild-type SVF cells (WT), NPGPx-knockout SVF cells (KO) and NPGPx-knockout SVF cells expressing human NPGPx (KO + hNPGPx) (left panels). Quantification of NBT reduction stains 8 days after induction (n = 3 per group) (right panels). *p = 0.0002 for WT vs. KO by independent Student's t-test.Oil Red O stain of WT, KO and KO + hNPGPx SVF (left panels). Quantification of Oil Red O stain 8 days after induction (n = 3 per group) (right panels). *p = 0.0002 for WT vs. KO by independent Student's t-test.Western blots showing ap2 and adiponectin expression in WT, KO and KO + hNPGPx SVF cells 8 days after induction.NBT reduction stain of NPGPx knockdown (KD) and control (V) 3T3-L1 preadipocytes after adipogenic induction (left panels). Quantification of NBT reduction stain at day 8 (n = 3 per group) (right panels). *p = 0.0004 by independent Student's t-test.Oil Red O staining of KD and V 3T3-L1 preadipocytes after adipogenic induction (left panels). Quantification of Oil Red O stain at day 8 (n = 3 per group) (right panels). *p = 0.0005 by independent Student's t-test.Western blots showing ap2 and adiponectin expression in NPGPx KD and V 3T3-L1 preadipocytes after adipogenic stimulation.NBT reduction stain and Oil Red O stain of NPGPx KD and V 3T3-L1 cells 8 days after induction. Cells were treated at with NAC at various doses the first 2 days after induction.Quantification of NBT reduction stain and Oil Red O stain in (K) (n = 3 per group).Correlation (R = 0.96) between ROS levels (Rel. NBT staining) and triglyceride contents (Rel. Oil-Red-O staining) in NPGPx KD and V 3T3-L1 cells 8 days after induction.Quantification of Oil Red O staining of NPGPx KD and V 3T3-L1 cells 8 days after induction. Cells were treated with H2O2 at different concentrations the first 2 days after induction (n = 3 per group). *p = 0.006, **p = 0.006, #p < 0.0001, ##p < 0.0001 by independent Student's t-test. All values are presented as means ± S.E.M. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23828861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse FABP4/A-FABP by Western Blot View Larger

Detection of Mouse FABP4/A-FABP by Western Blot A-FABP accelerates proteasomal degradation of LXR alpha.(a) Primary adipocytes derived from male 6-week-old A-FABP KO mice or WT littermates were treated with actinomycin D (actD, 1 μg ml−1) or vehicle (PBS). The mRNA level of LXR alpha was determined by real-time PCR at time points as indicated (n=4). (b,c) Primary brown adipocytes derived from male 6-week-old A-FABP KO mice or WT littermates were treated with (b) cycloheximide (CHX, 50 μg ml−1) or (c) CHX (50 μg ml−1) together with MG132 (10 μM) for different periods were subjected to immunoblotting using an antibody against LXR alpha, A-FABP and GADPH as indicated (n=4). (d) Primary brown adipocytes derived male 6-week-old A-FABP KO mice or WT littermates were infected with adenovirus overexpressing A-FABP (Ad-AFABP) or luciferase (Ad-Luci) for 48 h, followed by treatment with CHX (50 μg ml−1) for 0,6,12 and 24 h, and then subjected to immunoblotting using an antibody against LXR alpha, A-FABP and GADPH as indicated (n=4). The right panel is the band intensity of LXR alpha normalized with GAPDH, and expressed as percentage relative to baseline (0 h). Uncropped western blot images are shown in Supplementary Fig. 14. Data are represented as mean±s.e.m. *P<0.05, **P<0.01 (Students' t-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28128199), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse FABP4/A-FABP by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse FABP4/A-FABP by Immunocytochemistry/ Immunofluorescence Chemotherapy leads to fat loss in diet induced obese miceA. Weight of epididymal white adipose tissue (eWAT) from LFD, HFD, CY200 and MTX50 treated mice 1 week after last chemotherapy treatment. Each treatment group consisted of 6 mice, showing mean ± SD, data representative of 2 biological replicates. Comparisons by Student's t-test: ns - not significant, * p<0.02, ** p<0.004, *** p<0.002, **** p<0.0007 B. Representative illustration of abdominal cavities measured in (A), showing that large visceral fat deposits (arrows) were reduced post chemotherapy in HFD mice. C. Relative percent frequency of eWAT-associated adipocyte stem-like cells (ASC) in HFD mice treated with MTX12.5 or CY100. Each treatment group consisted of 3 mice, showing mean ± SD, data representative of 2 biological replicates. Statistics as in (A). D. Representative confocal imagery of sorted ASC cultured in adipogenic medium displaying lipid droplets. DAPI (blue)- DNA nuclear stain, FABP4 (green)- lipid transport protein in adipocytes, LipidTOX (red)- neutral lipid stain. Image collected and cropped by CiteAb from the following open publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.14576), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine FABP4/A-FABP by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine FABP4/A-FABP by Immunocytochemistry/ Immunofluorescence In vitro differentiation capacity of young and old BM-MSCs: (A) Representative micrographs from each experimental group showing morphological change after 21 days of differentiation. (B) Results of adipogenic, osteogenic and chondrogenic differentiation. Representative micrographs showing anti-FABP4, Oil Red O, anti-osteocalcin, Alizarin Red-S and anti-aggrecan staining in cultured BM-MSCs from each experimental group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21992089), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP4/A-FABP

FABP-4 (fatty acid binding protein-4; also Adipocyte-type FABP, ALBP and aP2) is a 14-15 kDa member of the fatty acid binding protein family, calycin superfamily of molecules.  It is expressed by adipocytes, macrophages, monocyte-derived DC and myoepithelial cells, and is known to serve as a carrier for select PPAR gamma fatty acid ligands as they enter the nucleus.  These ligands, or fatty acids, facilitate PPAR gamma -mediated gene transcription.  Mouse FABP-4 is 132 amino acids (aa) in length. It is a two beta -sheet molecule that contains an NLS (aa 22-32) and a fatty acid binding segment (aa 127-129).  Mouse FABP-4 is 94% and 92% aa identical to rat and human FABP-4, respectively.     

Long Name
Fatty Acid-Binding Protein 4
Entrez Gene IDs
2167 (Human); 11770 (Mouse); 79451 (Rat)
Alternate Names
Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte

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Citations for Mouse/Rat FABP4/A-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Combined Mek inhibition and Pparg activation Eradicates Muscle Invasive Bladder cancer in a Mouse Model of BBN-induced Carcinogenesis
    Authors: Tate, T;Plumber, SA;Al-Ahmadie, H;Chen, X;Choi, W;Lu, C;Viny, A;Batourina, E;Gartensson, K;Alija, B;Molotkov, A;Wiessner, G;McKiernan, J;McConkey, D;Dinney, C;Czerniak, B;Mendelsohn, CL;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Inflammatory signals from fatty bone marrow support DNMT3A driven clonal hematopoiesis
    Authors: N Zioni, AA Bercovich, N Chapal-Ila, T Bacharach, N Rappoport, A Solomon, R Avraham, E Kopitman, Z Porat, M Sacma, G Hartmut, M Scheller, C Muller-Tid, D Lipka, E Shlush, M Minden, N Kaushansky, LI Shlush
    Nature Communications, 2023-04-12;14(1):2070.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Early enforcement of cell identity by a functional component of the terminally differentiated state
    Authors: Z Bahrami-Ne, ZB Zhang, S Tholen, S Sharma, A Rabiee, ML Zhao, FB Kraemer, MN Teruel
    PloS Biology, 2022-12-05;20(12):e3001900.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  4. The circadian clock mediates daily bursts of cell differentiation by periodically restricting cell-differentiation commitment
    Authors: ZB Zhang, J Sinha, Z Bahrami-Ne, MN Teruel
    Proceedings of the National Academy of Sciences of the United States of America, 2022-08-08;119(33):e2204470119.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: ICC
  5. 1,2,3,4,6-Penta-O-galloyl-d-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity
    Authors: AR Sathyanara, CK Lu, CC Liaw, CC Chang, HY Han, BD Green, WJ Huang, C Huang, WD He, LC Lee, HK Liu
    International Journal of Molecular Sciences, 2022-04-06;23(7):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence
    Authors: Q Heydt, C Xintaropou, A Clear, M Austin, I Pislariu, F Miraki-Mou, P Cutillas, K Korfi, M Calaminici, W Cawthorn, K Suchacki, A Nagano, JG Gribben, M Smith, JD Cavenagh, H Oakervee, A Castleton, D Taussig, B Peck, A Wilczynska, L McNaughton, D Bonnet, F Mardakheh, B Patel
    Nature Communications, 2021-09-17;12(1):5507.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  7. Fatty acid binding protein-4 promotes autoimmune diabetes by recruitment and activation of pancreatic islet macrophages
    Authors: Y Xiao, L Shu, X Wu, Y Liu, LY Cheong, B Liao, X Xiao, RL Hoo, Z Zhou, A Xu
    JCI Insight, 2021-04-08;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
    Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
    Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  9. Adipocyte Fatty Acid Transfer Supports Megakaryocyte Maturation
    Authors: C Valet, A Batut, A Vauclard, A Dortignac, M Bellio, B Payrastre, P Valet, S Severin
    Cell Rep, 2020-07-07;32(1):107875.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Molecular Competition in G1 Controls When Cells Simultaneously Commit to Terminally Differentiate and Exit the Cell Cycle
    Authors: ML Zhao, A Rabiee, KM Kovary, Z Bahrami-Ne, B Taylor, MN Teruel
    Cell Rep, 2020-06-16;31(11):107769.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Haematopoietic stem cells in perisinusoidal niches are protected from ageing
    Authors: M Saçma, J Pospiech, R Bogeska, W de Back, JP Mallm, V Sakk, K Soller, G Marka, A Vollmer, R Karns, N Cabezas-Wa, A Trumpp, S Méndez-Fer, MD Milsom, MA Mulaw, H Geiger, MC Florian
    Nat. Cell Biol., 2019-11-04;21(11):1309-1320.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  12. Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells
    Authors: C Liu, T Tate, E Batourina, ST Truschel, S Potter, M Adam, T Xiang, M Picard, M Reiley, K Schneider, M Tamargo, C Lu, X Chen, J He, H Kim, CL Mendelsohn
    Nat Commun, 2019-10-09;10(1):4589.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems
    Authors: V Miceli, M Pampalone, S Vella, AP Carreca, G Amico, PG Conaldi
    Stem Cells Int, 2019-02-18;2019(0):7486279.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds
    Authors: CF Guerrero-J, PH Dedhia, S Jin, R Ruiz-Vega, D Ma, Y Liu, K Yamaga, O Shestova, DL Gay, Z Yang, K Kessenbroc, Q Nie, WS Pear, G Cotsarelis, MV Plikus
    Nat Commun, 2019-02-08;10(1):650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Maintenance of the bladder cancer precursor urothelial hyperplasia requires FOXA1 and persistent expression of oncogenic HRAS
    Authors: CH Yee, Z Zheng, L Shuman, H Yamashita, JI Warrick, XR Wu, JD Raman, DJ DeGraff
    Sci Rep, 2019-01-22;9(1):270.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes
    Authors: L Shu, RL Hoo, X Wu, Y Pan, IP Lee, LY Cheong, SR Bornstein, X Rong, J Guo, A Xu
    Nat Commun, 2017-01-27;8(0):14147.
    Species: Mouse
    Sample Types: Serum, Tissue Homogenates
    Applications: Immunoprecipitation, Western Blot
  17. Chemotherapy can induce weight normalization of morbidly obese mice despite undiminished ingestion of high fat diet
    Authors: CE Myers, DB Hoelzinger, TN Truong, LA Chew, A Myles, L Chaudhuri, JB Egan, J Liu, SJ Gendler, PA Cohen
    Oncotarget, 2017-01-17;8(3):5426-5438.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. Epigenetic programming of Dnmt3a mediated by AP2? is required for granting preadipocyte the ability to differentiate
    Cell Death Dis, 2016-12-01;7(12):e2496.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Epidermal Wnt/beta-catenin signaling regulates adipocyte differentiation via secretion of adipogenic factors.
    Authors: Donati G, Proserpio V, Lichtenberger B, Natsuga K, Sinclair R, Fujiwara H, Watt F
    Proc Natl Acad Sci U S A, 2014-03-31;111(15):E1501-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013-12-12;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. Artificial sweeteners stimulate adipogenesis and suppress lipolysis independently of sweet taste receptors.
    Authors: Simon B, Parlee S, Learman B, Mori H, Scheller E, Cawthorn W, Ning X, Gallagher K, Tyrberg B, Assadi-Porter F, Evans C, MacDougald O
    J Biol Chem, 2013-09-24;288(45):32475-89.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.
    Authors: Asumda FZ, Chase PB
    BMC Cell Biol., 2011-10-12;12(0):44.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  23. Cyclooxygenase-2 deficiency attenuates adipose tissue differentiation and inflammation in mice.
    Authors: Ghoshal S, Trivedi DB, Graf GA, Loftin CD
    J. Biol. Chem., 2010-10-20;286(1):889-98.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Goat IgG (H+L) Antibody

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