Mouse/Rat FABP4/A-FABP Antibody

Catalog # Availability Size / Price Qty
AF1443
AF1443-SP
Mouse/Rat FABP4/A-FABP Antibody in Data
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Product Details
Citations (12)
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Mouse/Rat FABP4/A-FABP Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse FABP4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) FABP5 and rmFABP9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse FABP4
Met1-Ala132
Accession # P04117
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse FABP4/A‑FABP by Western Blot. View Larger

Detection of Mouse FABP4/A‑FABP by Western Blot. Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry FABP4 in Mouse Adipocytes. View Larger

FABP4 in Mouse Adipocytes. FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry FABP4 in Rat Mesenchymal Stem Cells. View Larger

FABP4 in Rat Mesenchymal Stem Cells. FABP4 was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry FABP4 in Mouse Intestine. View Larger

FABP4 in Mouse Intestine. FABP4 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse FABP4/A‑FABP by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse FABP4/A‑FABP by Simple WesternTM. Simple Western lane view shows lysates of mouse thymus tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A-FABP at approximately 17 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse/Rat FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP4/A-FABP

FABP-4 (fatty acid binding protein-4; also Adipocyte-type FABP, ALBP and aP2) is a 14-15 kDa member of the fatty acid binding protein family, calycin superfamily of molecules.  It is expressed by adipocytes, macrophages, monocyte-derived DC and myoepithelial cells, and is known to serve as a carrier for select PPAR gamma fatty acid ligands as they enter the nucleus.  These ligands, or fatty acids, facilitate PPAR gamma -mediated gene transcription.  Mouse FABP-4 is 132 amino acids (aa) in length. It is a two beta -sheet molecule that contains an NLS (aa 22-32) and a fatty acid binding segment (aa 127-129).  Mouse FABP-4 is 94% and 92% aa identical to rat and human FABP-4, respectively.     

Long Name
Fatty Acid-Binding Protein 4
Entrez Gene IDs
2167 (Human); 11770 (Mouse); 79451 (Rat)
Alternate Names
Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte

Product Datasheets

Citations for Mouse/Rat FABP4/A-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Maintenance of the bladder cancer precursor urothelial hyperplasia requires FOXA1 and persistent expression of oncogenic HRAS
    Authors: CH Yee, Z Zheng, L Shuman, H Yamashita, JI Warrick, XR Wu, JD Raman, DJ DeGraff
    Sci Rep, 2019;9(1):270.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds
    Authors: CF Guerrero-J, PH Dedhia, S Jin, R Ruiz-Vega, D Ma, Y Liu, K Yamaga, O Shestova, DL Gay, Z Yang, K Kessenbroc, Q Nie, WS Pear, G Cotsarelis, MV Plikus
    Nat Commun, 2019;10(1):650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems
    Authors: V Miceli, M Pampalone, S Vella, AP Carreca, G Amico, PG Conaldi
    Stem Cells Int, 2019;2019(0):7486279.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells
    Authors: C Liu, T Tate, E Batourina, ST Truschel, S Potter, M Adam, T Xiang, M Picard, M Reiley, K Schneider, M Tamargo, C Lu, X Chen, J He, H Kim, CL Mendelsohn
    Nat Commun, 2019;10(1):4589.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes
    Authors: L Shu, RL Hoo, X Wu, Y Pan, IP Lee, LY Cheong, SR Bornstein, X Rong, J Guo, A Xu
    Nat Commun, 2017;8(0):14147.
    Species: Mouse
    Sample Types: Serum
    Applications: Immunoprecipitation
  6. Chemotherapy can induce weight normalization of morbidly obese mice despite undiminished ingestion of high fat diet
    Authors: CE Myers, DB Hoelzinger, TN Truong, LA Chew, A Myles, L Chaudhuri, JB Egan, J Liu, SJ Gendler, PA Cohen
    Oncotarget, 2017;8(3):5426-5438.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Epigenetic programming of Dnmt3a mediated by AP2? is required for granting preadipocyte the ability to differentiate
    Cell Death Dis, 2016;7(12):e2496.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Epidermal Wnt/beta-catenin signaling regulates adipocyte differentiation via secretion of adipogenic factors.
    Authors: Donati G, Proserpio V, Lichtenberger B, Natsuga K, Sinclair R, Fujiwara H, Watt F
    Proc Natl Acad Sci U S A, 2014;111(15):E1501-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Artificial sweeteners stimulate adipogenesis and suppress lipolysis independently of sweet taste receptors.
    Authors: Simon B, Parlee S, Learman B, Mori H, Scheller E, Cawthorn W, Ning X, Gallagher K, Tyrberg B, Assadi-Porter F, Evans C, MacDougald O
    J Biol Chem, 2013;288(45):32475-89.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.
    Authors: Asumda FZ, Chase PB
    BMC Cell Biol., 2011;12(0):44.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  12. Cyclooxygenase-2 deficiency attenuates adipose tissue differentiation and inflammation in mice.
    Authors: Ghoshal S, Trivedi DB, Graf GA, Loftin CD
    J. Biol. Chem., 2011;286(1):889-98.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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