Mouse ST2/IL-33 R Antibody

Catalog # Availability Size / Price Qty
AF1004
AF1004-SP
Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody.
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Product Details
Citations (5)
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Mouse ST2/IL-33 R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ST2/IL-33 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R
Ser27-Arg332
Accession # P14719
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse ST2/IL-33 R Fc Chimera (Catalog # 1004-MR)
Neutralization
Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 1-6 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33 and Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody. View Larger

Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody. Recombinant Human IL‑33 (Catalog # 3625-IL) induces proliferation in the D10.G4.1 mouse helper T cell line in the presence of Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL‑33 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004). The ND50 is typically
1-6 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST2/IL-33 R

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses. Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain. Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions. Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts. IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL‑1 R rp2. In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal. In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy. The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma. Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2.

Long Name
Interleukin 33 Receptor
Entrez Gene IDs
9173 (Human); 17082 (Mouse)
Alternate Names
DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1

Product Datasheets

Citations for Mouse ST2/IL-33 R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. alpha-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3.
    Authors: Huang J, Tsai Y, Chang Y, Wu J, Hung J, Lin K, Wong C, Yu A
    J Immunol, 2014;192(4):1972-81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neut
  2. Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
    Authors: Mizutani N, Nabe T, Yoshino S
    Immunology, 2013;139(2):205-18.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  3. IL-33 is produced by mast cells and regulates IgE-dependent inflammation.
    Authors: Hsu CL, Neilsen CV, Bryce PJ
    PLoS ONE, 2010;5(8):e11944.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  4. Protective effect of IL-18 on kainate- and IL-1beta-induced cerebellar ataxia in mice.
    Authors: Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A
    J. Immunol., 2008;180(4):2322-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  5. ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis.
    Authors: Huang X, Du W, Barrett RP, Hazlett LD
    Invest. Ophthalmol. Vis. Sci., 2007;48(10):4626-33.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

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