Mouse Thrombomodulin/BDCA-3 Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Thrombomodulin/BDCA-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
    2% cross‑reactivity with recombinant human Thrombomodulin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Thrombomodulin/BDCA-3
    Leu17-Ser517
    Accession # P15306
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Thrombomodulin/BDCA-3 (Catalog # 3894-PA)
  • Flow Cytometry
    2.5 µg/106 cells
    bEnd.3 mouse endothelioma cell line
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Mouse Thrombomodulin/BDCA-3 (Catalog # 3894-PA), see our available Western blot detection antibodies
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Thrombomodulin/BDCA‑3 in bEnd.3 Mouse Cell Line. Thrombomodulin/BDCA‑3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Thrombomodulin/BDCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3894) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombomodulin/BDCA-3

Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid sequence of mouse THBD predicts a signal peptide (aa 1 to 16) and a mature chain (aa 17 to 577) that consists of the following domains: C-type lectin (aa 31 to 167), EGF-like (aa 240 to 280, aa 283 to 323, aa 324 to 362, aa 364 to 404, aa 405 to 439, and aa 440 to 480), transmembrane (aa 518 to 541) and cytoplasmic (aa 542 to 577) (1). The R&D Systems recombinant mouse THBD consists of aa 17 to 517, corresponding to the extracellular portion of the type I membrane protein.

Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (2‑4). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (5). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (6).

  • References:
    1. Dittman, W.A. and P.W. Majerus (1989) Nucleic Acids Res. 17:802.
    2. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
    3. Wu, K.K. et al. (2000) Ann Med. 32:73.
    4. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
    5. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
    6. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.
  • Entrez Gene IDs:
    7056 (Human); 21824 (Mouse); 83580 (Rat)
  • Alternate Names:
    BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia
    Authors: BM Nagy, C Nagaraj, B Egemnazaro, G Kwapiszews, RE Stauber, A Avian, H Olschewski, A Olschewski
    Front Physiol, 2017;8(0):98.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor.
    Authors: Greineder, Colin F, Chacko, Ann-Mari, Zaytsev, Sergei, Zern, Blaine J, Carnemolla, Ronald, Hood, Elizabet, Han, Jingyan, Ding, Bi-Sen, Esmon, Charles, Muzykantov, Vladimir
    PLoS ONE, 2013;8(11):e80110.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.
    Nat. Med., 2012;18(0):1217.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling.
    Authors: Graham BB, Mentink-Kane MM, El-Haddad H
    Am. J. Pathol., 2010;177(3):1549-61.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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