Mouse TRANCE/TNFSF11/RANK L Antibody

(11 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TRANCE/TNFSF11/RANK L in ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) CD27, rmCD40, and rmFas is observed and less than 1% cross-reactivity with rmCD30, recombinant human (rh) DR6, rmGITR, rmTNF RI, and rmTNF RII is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TRANCE/TNFSF11/RANK L
    Arg72-Asp316
    Accession # O35235
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR)
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse thymus
    • Mouse TRANCE/TNFSF11/RANK L Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse TRANCE/TNFSF11/RANK L Antibody (Catalog # AF462)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody (Catalog # BAF462)
  • ELISA Standard
     
    Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein (Catalog # 462-TR)
  • Neutralization
    Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in mouse splenocytes. Nakagawa, N. et al. (1998) Biochim. Biophys. Res. Commun. 253:395. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 30 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Osteoclast-like Cell Formation Induced by TRANCE/
TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody.
In the presence of Recombinant Mouse M‑CSF (20 ng/mL, Catalog # 416-ML), Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF11/
RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF11/RANK L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF462). The ND50 is typically 0.02-0.06 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRANCE/TNFSF11/RANK L

TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

  • References:
    1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
    2. Anderson, D.M. et al. (1997) Nature 390:175.
    3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
    4. Kong, Y-Y. et al. (1999) Nature 397:315.
  • Long Name:
    TNF-related Activation-induced Cytokine
  • Entrez Gene IDs:
    8600 (Human); 21943 (Mouse); 117516 (Rat)
  • Alternate Names:
    CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis
    Authors: X Zhang, HC Chiang, Y Wang, C Zhang, S Smith, X Zhao, SJ Nair, J Michalek, I Jatoi, M Lautner, B Oliver, H Wang, A Petit, T Soler, J Brunet, F Mateo, M Angel Puja, E Poggi, K Chaldekas, C Isaacs, BN Peshkin, O Ochoa, F Chedin, C Theoharis, LZ Sun, TJ Curiel, R Elledge, VX Jin, Y Hu, R Li
    Nat Commun, 2017;8(0):15908.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity.
    Authors: Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V
    Autophagy, 2014;10(11):2036-52.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  3. Fibromodulin and Biglycan Modulate Periodontium through TGFbeta/BMP Signaling.
    Authors: Wang L, Foster B, Kram V, Nociti F, Zerfas P, Tran A, Young M, Somerman M
    J Dent Res, 2014;93(8):780-787.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
    Authors: Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
    PLoS ONE, 2014;9(5):e96286.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  5. CXCL5 stimulation of RANK ligand expression in Paget's disease of bone.
    Authors: Sundaram K, Rao D, Ries W, Reddy S
    Lab Invest, 2013;93(4):472-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer.
    Authors: Schramek D, Leibbrandt A, Sigl V, Kenner L, Pospisilik JA, Lee HJ, Hanada R, Joshi PA, Aliprantis A, Glimcher L, Pasparakis M, Khokha R, Ormandy CJ, Widschwendter M, Schett G, Penninger JM
    Nature, 2010;468(7320):98-102.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse.
    Authors: Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP
    Dev. Biol., 2009;328(1):127-39.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice.
    Authors: Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL
    Circulation, 2008;117(3):411-20.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Estrogen and progesterone are critical regulators of Stat5a expression in the mouse mammary gland.
    Authors: Santos SJ, Haslam SZ, Conrad SE
    Endocrinology, 2007;149(1):329-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
    Authors: Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB
    J. Immunol., 2003;170(5):2655-62.
    Species: Mouse
    Sample Type: Synovial Fluid
    Application: ELISA Development
  11. Paracrine-rescued lobulogenesis in chimeric outgrowths comprising progesterone-receptor-null mammary epithelium and redirected wild-type testicular cells.
    Authors: Bruno R, Boulanger C, Rosenfield S, Anderson L, Lydon J, Smith G
    J Cell Sci, 0;127(0):27-32.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
Expand to show all 11 Citations
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