Mouse TRANCE/TNFSF11/RANK L Antibody

Catalog # Availability Size / Price Qty
AF462
AF462-SP
Mouse TRANCE/TNFSF11/RANK L Antibody in Data
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Citations (16)
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Mouse TRANCE/TNFSF11/RANK L Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TRANCE/TNFSF11/RANK L in ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) CD27, rmCD40, and rmFas is observed and less than 1% cross-reactivity with rmCD30, recombinant human (rh) DR6, rmGITR, rmTNF RI, and rmTNF RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TRANCE/TNFSF11/RANK L
Arg72-Asp316
Accession # O35235
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse thymus

Mouse TRANCE/TNFSF11/RANK L Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody (Catalog # BAF462)

Standard: Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein (Catalog # 462-TR)

Neutralization
Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in mouse splenocytes. Nakagawa, N. et al. (1998) Biochim. Biophys. Res. Commun. 253:395. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 30 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody. View Larger

Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M-CSF (20 ng/mL, Catalog # 416-ML), Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF11/ RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF11/RANK L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF462). The ND50 is typically 0.02-0.06 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

References
  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.
Long Name
TNF-related Activation-induced Cytokine
Entrez Gene IDs
8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)
Alternate Names
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11

Product Datasheets

Citations for Mouse TRANCE/TNFSF11/RANK L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Antiresorptive activity of osteoprotegerin requires an intact heparan sulfate-binding site
    Authors: M Li, D Xu
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer
    Authors: R Pascual, J Martín, F Salvador, O Reina, V Chanes, A Millanes-R, C Suñer, G Fernández-, A Bartomeu, YS Huang, RR Gomis, R Méndez
    Sci Adv, 2020;6(20):eaax3868.
    Species: Human
    Sample Types: Tissue
    Applications: IHC
  3. Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence
    Authors: HN Kim, J Xiong, RS MacLeod, S Iyer, Y Fujiwara, KM Cawley, L Han, Y He, JD Thostenson, E Ferreira, RL Jilka, D Zhou, M Almeida, CA O'Brien
    JCI Insight, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  4. RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis
    Authors: J Jhun, SH Lee, SY Kim, J Ryu, JY Kwon, HS Na, K Jung, SJ Moon, ML Cho, JK Min
    J Transl Med, 2019;17(1):84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Soluble RANKL contributes to osteoclast formation in adult mice but not ovariectomy-induced bone loss
    Authors: J Xiong, K Cawley, M Piemontese, Y Fujiwara, H Zhao, JJ Goellner, CA O'Brien
    Nat Commun, 2018;9(1):2909.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  6. Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis
    Authors: X Zhang, HC Chiang, Y Wang, C Zhang, S Smith, X Zhao, SJ Nair, J Michalek, I Jatoi, M Lautner, B Oliver, H Wang, A Petit, T Soler, J Brunet, F Mateo, M Angel Puja, E Poggi, K Chaldekas, C Isaacs, BN Peshkin, O Ochoa, F Chedin, C Theoharis, LZ Sun, TJ Curiel, R Elledge, VX Jin, Y Hu, R Li
    Nat Commun, 2017;8(0):15908.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Fibromodulin and Biglycan Modulate Periodontium through TGFbeta/BMP Signaling.
    Authors: Wang L, Foster B, Kram V, Nociti F, Zerfas P, Tran A, Young M, Somerman M
    J Dent Res, 2014;93(8):780-787.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
    Authors: Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
    PLoS ONE, 2014;9(5):e96286.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity.
    Authors: Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V
    Autophagy, 2014;10(11):2036-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. CXCL5 stimulation of RANK ligand expression in Paget's disease of bone.
    Authors: Sundaram K, Rao D, Ries W, Reddy S
    Lab Invest, 2013;93(4):472-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer.
    Authors: Schramek D, Leibbrandt A, Sigl V, Kenner L, Pospisilik JA, Lee HJ, Hanada R, Joshi PA, Aliprantis A, Glimcher L, Pasparakis M, Khokha R, Ormandy CJ, Widschwendter M, Schett G, Penninger JM
    Nature, 2010;468(7320):98-102.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse.
    Authors: Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP
    Dev. Biol., 2009;328(1):127-39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice.
    Authors: Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL
    Circulation, 2008;117(3):411-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Estrogen and progesterone are critical regulators of Stat5a expression in the mouse mammary gland.
    Authors: Santos SJ, Haslam SZ, Conrad SE
    Endocrinology, 2007;149(1):329-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
    Authors: Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB
    J. Immunol., 2003;170(5):2655-62.
    Species: Mouse
    Sample Types: Synovial Fluid
    Applications: ELISA Development
  16. Paracrine-rescued lobulogenesis in chimeric outgrowths comprising progesterone-receptor-null mammary epithelium and redirected wild-type testicular cells.
    Authors: Bruno R, Boulanger C, Rosenfield S, Anderson L, Lydon J, Smith G
    J Cell Sci, 0;127(0):27-32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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Mouse TRANCE/TNFSF11/RANK L Antibody
By Anonymous on 07/06/2021
Application: ICC/IF Sample Tested: Bone Species: Mouse