Rabbit IL-6 DuoSet ELISA
Rabbit IL-6 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Data Example
Product Datasheets
Preparation and Storage
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
Citations for Rabbit IL-6 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Implant delivering hydroxychloroquine attenuates vaginal T lymphocyte activation and inflammation
Authors: Y Chen, YL Traore, S Yang, J Lajoie, KR Fowke, DW Rickey, EA Ho
J Control Release, 2018;0(0):.
Species: Rabbit
Sample Types: Cervicovaginal Fluid
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Salusin-? Inhibits Proliferation and Migration of Vascular Smooth Muscle Cell via Akt/mTOR Signaling
Authors: S Gao, L Xu, Y Zhang, Q Yu, J Li, H Guan, X Wang, D Cheng, Y Liu, L Bai, R Wang, J Fan, S Zhao, E Liu
Cell. Physiol. Biochem., 2018;50(5):1740-1753.
Species: Rabbit
Sample Types: Plasma
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Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3
Authors: X Li, C Tang, J Wang, P Guo, C Wang, Y Wang, Z Zhang, H Wu
Exp Ther Med, 2018;15(4):3856-3864.
Species: Rabbit
Sample Types: Tissue Homogenates
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Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study
Sci Rep, 2016;6(0):33657.
Species: Rabbit
Sample Types: Serum
FAQs
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