Rabbit IL-6 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Data Example
Product Datasheets
Preparation and Storage
Background: IL-6
Interleukin 6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells (2, 11-33). IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids (2).
Citations for Rabbit IL-6 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Therapeutic potential of fibrinogen &gamma-chain peptide-coated, ADP-encapsulated liposomes as a haemostatic adjuvant for post-cardiopulmonary bypass coagulopathy
Authors: O Ishida, K Hagisawa, N Yamanaka, K Tsutsumi, H Suzuki, M Takikawa, S Takeoka, M Kinoshita
Sci Rep, 2020;10(1):11308.
Species: Rabbit
Sample Types: Plasma
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Implant delivering hydroxychloroquine attenuates vaginal T lymphocyte activation and inflammation
Authors: Y Chen, YL Traore, S Yang, J Lajoie, KR Fowke, DW Rickey, EA Ho
J Control Release, 2018;0(0):.
Species: Rabbit
Sample Types: Cervicovaginal Fluid
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Salusin-? Inhibits Proliferation and Migration of Vascular Smooth Muscle Cell via Akt/mTOR Signaling
Authors: S Gao, L Xu, Y Zhang, Q Yu, J Li, H Guan, X Wang, D Cheng, Y Liu, L Bai, R Wang, J Fan, S Zhao, E Liu
Cell. Physiol. Biochem., 2018;50(5):1740-1753.
Species: Rabbit
Sample Types: Plasma
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Methylene blue relieves the development of osteoarthritis by upregulating lncRNA MEG3
Authors: X Li, C Tang, J Wang, P Guo, C Wang, Y Wang, Z Zhang, H Wu
Exp Ther Med, 2018;15(4):3856-3864.
Species: Rabbit
Sample Types: Tissue Homogenates
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Effects of Methotrexate in a Rabbit Model of In-Stent Neoatherosclerosis: An Optical Coherence Tomography Study
Sci Rep, 2016;6(0):33657.
Species: Rabbit
Sample Types: Serum
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