Rat Adiponectin/Acrp30 Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat Adiponectin/Acrp30 in direct ELISAs and Western blots. In Western blots, approximately 45% cross-reactivity with recombinant mouse Adiponectin is observed and 35% cross-reactivity with recombinant human Adiponectin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat Adiponectin/Acrp30
    Ile19-Asn244
    Accession # NP_653345
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat Adiponectin/Acrp30
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Adiponetin/Acrp30 in Rat Adipocytes. Adiponectin/Adipocyte complement-related protein of 30 kDa (ACRP-30) was detected in rat adipocytes using Rat Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
Adiponectin/Acrp30 in Rat Liver. Adiponectin/Acrp30 was detected in perfusion fixed frozen sections of rat liver using Rat Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3100) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunocytochemistry
Adiponectin/Acrp30 in Mouse Adipocytes. Adiponectin/Acrp30 was detected in immersion fixed mouse mesenchymal stem cells differentiated into adipocytes using Rat Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adiponectin/Acrp30

Adiponectin, also known as Adipocyte complement-related protein of 30 kDa (Acrp30), is a secreted adipose tissue-specific protein. It is an anti-atherogenic, anti-diabetic and anti-inflammatory hormone that plays important roles in the regulation of lipid and glucose metabolism. Rat Adiponectin/Acrp30 shares 91% and 83% amino acid sequence homology with mouse and human Adiponectin/Acrp30, respectively.

  • Entrez Gene IDs:
    9370 (Human); 11450 (Mouse); 246253 (Rat)
  • Alternate Names:
    ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
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