Rat GABAB R2 N-Terminus Antibody

Catalog # Availability Size / Price Qty
AF1188
AF1188-SP
    GABAB R2  in Rat Spinal Cord.
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Product Details
Citations (1)
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Rat GABAB R2 N-Terminus Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat GABAB R2 N-Terminus in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat GABAB R2
Trp41-Ser482 (Phe337Tyr)
Accession # O88871
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat GABAB R2 N-Terminus
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry GABAB R2  in Rat Spinal Cord. View Larger

GABAB R2 in Rat Spinal Cord. GABAB R2 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GABAB R2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GABA-B R2

The neurotransmitter gamma-aminobutyric acid (GABA) mediates an inhibitory signal upon binding to two ligand-gated channels, GABA-A and GABA-C receptors, and a G-protein linked receptor, GABA-B. The GABA-B receptor is a heterodimeric complex composed of two 7-transmembrane GPCRs termed GABA-B R1 and GABA-B R2. GABA-B receptors have been implicated in the GABA-induced modulation of sympathetic and catecholaminergic pathways in the central nervous system.

Long Name
gamma-Aminobutyric Acid Type B Receptor Subunit 2
Entrez Gene IDs
9568 (Human)
Alternate Names
G protein-coupled receptor 51; GABA B R2; GABABR2; GABA-BR2; GABA-B-R2; GABABR2GABA-B receptor 2; GABBR2; gamma-aminobutyric acid (GABA) B receptor, 2; gamma-aminobutyric acid type B receptor subunit 2; gb2; GPR51; GPR51GABA-B receptor, R2 subunit; GPRC3B; GPRC3BFLJ36928; G-protein coupled receptor 51; HG20HRIHFB2099

Product Datasheets

Citation for Rat GABAB R2 N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics.
    Authors: Schwenk J, Perez-Garci E, Schneider A, Kollewe A, Gauthier-Kemper A, Fritzius T, Raveh A, Dinamarca M, Hanuschkin A, Bildl W, Klingauf J, Gassmann M, Schulte U, Bettler B, Fakler B
    Nat Neurosci, 2016;19(2):233-42.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: WB

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