Rat GABA_B R2 N-Terminus Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry View Larger

GABA_BR2 in Rat Spinal Cord. GABA_BR2 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GABA_BR2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western View Larger

Detection of Rat GABA_B R2 by Simple Western^TM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GABA_B R2 at approximately 148 kDa (as indicated) using 20 µg/mL of Goat Anti-Rat GABA_B R2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Preparation and Storage

Background: GABA-B R2

GABBR2 is half of a heterodimeric GPCR for GABA which mediates coupling to G proteins. B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase As a homodimer, GABBR2 is retained in the endoplasmic reticulum and endoplasmic reticulum-Golgi intermediate compartment.  When GBR1/GBR2 heterodimers are present they are localized to the plasma membrane. GABBR2 is differentially expressed in the nervous system. GABBR2 and GABBR1 mRNA's are coexpressed in various brain regions such as the Purkinje cell layer of the cerebellum. In situ hybridization histochemistry using an antisensce probe to this novel receptor indicates GABBR2 is found exclusively in neurons. GABBR2 is implicated in synaptic inhibition, hippocampal long-term potentiation, slow wave sleep, muscle relaxation and nociception.

Product Datasheets

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