Rat GABA-B R2 N-Terminus Antibody

R&D Systems | Catalog # AF1188

R&D Systems

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GABA-B R2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat GABAB R2
Trp41-Ser482 (Phe337Tyr)
Accession # O88871

Specificity

Detects rat GABAB R2 N-Terminus in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat GABA-B R2 N-Terminus Antibody

GABABR2 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr).

GABABR2 in Rat Spinal Cord.

GABABR2 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GABABR2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Rat GABAB R2 antibody by Simple WesternTM.

Detection of Rat GABAB R2 by Simple WesternTM.

Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GABAB R2 at approximately 148 kDa (as indicated) using 20 µg/mL of Goat Anti-Rat GABAB R2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Rat GABA-B R2 N-Terminus Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cortex) and spinal cord

Simple Western

20 µg/mL
Sample: Rat brain tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Rat GABAB R2 N-Terminus

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GABA-B R2

GABBR2 is half of a heterodimeric GPCR for GABA which mediates coupling to G proteins. B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase As a homodimer, GABBR2 is retained in the endoplasmic reticulum and endoplasmic reticulum-Golgi intermediate compartment.  When GBR1/GBR2 heterodimers are present they are localized to the plasma membrane. GABBR2 is differentially expressed in the nervous system. GABBR2 and GABBR1 mRNA's are coexpressed in various brain regions such as the Purkinje cell layer of the cerebellum. In situ hybridization histochemistry using an antisensce probe to this novel receptor indicates GABBR2 is found exclusively in neurons. GABBR2 is implicated in synaptic inhibition, hippocampal long-term potentiation, slow wave sleep, muscle relaxation and nociception.

Long Name

gamma-Aminobutyric Acid Type B Receptor Subunit 2

Alternate Names

GABABR2, GABBR2, GPR51, GPRC3B

Entrez Gene IDs

9568 (Human); 83633 (Rat)

Gene Symbol

GABBR2

UniProt

O88871

Additional GABA-B R2 Products

Product Documents for Rat GABA-B R2 N-Terminus Antibody

Certificate of Analysis

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Product Specific Notices for Rat GABA-B R2 N-Terminus Antibody

For research use only

Citations for Rat GABA-B R2 N-Terminus Antibody

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Protocols

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