Rat GABAB R2 N-Terminus Antibody

Catalog # Availability Size / Price Qty
GABABR2 in Rat Spinal Cord.
2 Images
Product Details
Citations (1)
Supplemental Products

Rat GABAB R2 N-Terminus Antibody Summary

Species Reactivity
Detects rat GABAB R2 N-Terminus in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant rat GABAB R2
Trp41-Ser482 (Phe337Tyr)
Accession # O88871
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Rat GABAB R2 N-Terminus
Simple Western
20 µg/mL
Rat brain tissue
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GABA<sub>B</sub>R2 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr). View Larger

GABABR2 in Rat Spinal Cord. GABABR2 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GABABR2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Rat GABA<sub>B</sub> R2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Rat GABAB R2 by Simple WesternTM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GABAB R2 at approximately 148 kDa (as indicated) using 20 µg/mL of Goat Anti-Rat GABAB R2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GABA-B R2

GABBR2 is half of a heterodimeric GPCR for GABA which mediates coupling to G proteins. B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase As a homodimer, GABBR2 is retained in the endoplasmic reticulum and endoplasmic reticulum-Golgi intermediate compartment.  When GBR1/GBR2 heterodimers are present they are localized to the plasma membrane. GABBR2 is differentially expressed in the nervous system. GABBR2 and GABBR1 mRNA's are coexpressed in various brain regions such as the Purkinje cell layer of the cerebellum. In situ hybridization histochemistry using an antisensce probe to this novel receptor indicates GABBR2 is found exclusively in neurons. GABBR2 is implicated in synaptic inhibition, hippocampal long-term potentiation, slow wave sleep, muscle relaxation and nociception.

Long Name
gamma-Aminobutyric Acid Type B Receptor Subunit 2
Entrez Gene IDs
9568 (Human); 83633 (Rat)
Alternate Names
G protein-coupled receptor 51; GABA B R2; GABABR2; GABA-BR2; GABA-B-R2; GABABR2GABA-B receptor 2; GABBR2; gamma-aminobutyric acid (GABA) B receptor, 2; gamma-aminobutyric acid type B receptor subunit 2; gb2; GPR51; GPR51GABA-B receptor, R2 subunit; GPRC3B; GPRC3BFLJ36928; G-protein coupled receptor 51; HG20HRIHFB2099

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Citation for Rat GABAB R2 N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics.
    Authors: Schwenk J, Perez-Garci E, Schneider A, Kollewe A, Gauthier-Kemper A, Fritzius T, Raveh A, Dinamarca M, Hanuschkin A, Bildl W, Klingauf J, Gassmann M, Schulte U, Bettler B, Fakler B
    Nat Neurosci, 2016;19(2):233-42.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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