Rat GABAB R2 N-Terminus Antibody Summary
Accession # O88871
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
GABABR2 in Rat Spinal Cord. GABABR2 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GABABR2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Rat GABAB R2 by Simple WesternTM.
Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GABAB R2 at approximately 148 kDa (as indicated) using 20 µg/mL of Goat Anti-Rat GABAB R2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GABA-B R2
The neurotransmitter gamma-aminobutyric acid (GABA) mediates an inhibitory signal upon binding to two ligand-gated channels, GABA-A and GABA-C receptors, and a G-protein linked receptor, GABA-B. The GABA-B receptor is a heterodimeric complex composed of two 7-transmembrane GPCRs termed GABA-B R1 and GABA-B R2. GABA-B receptors have been implicated in the GABA-induced modulation of sympathetic and catecholaminergic pathways in the central nervous system.
Citation for Rat GABAB R2 N-Terminus Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics.
Authors: Schwenk J, Perez-Garci E, Schneider A, Kollewe A, Gauthier-Kemper A, Fritzius T, Raveh A, Dinamarca M, Hanuschkin A, Bildl W, Klingauf J, Gassmann M, Schulte U, Bettler B, Fakler B
Nat Neurosci, 2016;19(2):233-42.
Sample Types: Cell Lysates
Applications: Western Blot
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