Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 222517
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Product Specifications

Immunogen

E. coli-derived recombinant rat Growth Hormone
Phe27-Phe216
Accession # P01244

Specificity

Detects rat Growth Hormone in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Growth Hormone is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Rat Growth Hormone Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat pituitary

Western Blot

1 µg/mL
Sample: Recombinant Rat Growth Hormone

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Growth Hormone

Growth Hormone, also known as somatotropin, is synthesized primarily in the anterior pituitary. It is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor which is present in many cell types.

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Entrez Gene IDs

2688 (Human)

Gene Symbol

GH1

UniProt

Additional Growth Hormone Products

Product Documents for Rat Growth Hormone Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat Growth Hormone Antibody

For research use only

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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