Rat IL-4 Antibody Summary
Accession # P20096
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑4 and Neutralization by Rat IL‑4 Antibody. Recombinant Rat IL-4 (Catalog # 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-4 (4 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA). The ND50 is typically 0.05-015 µg/mL in the presence of PHA (10 µg/mL).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rat IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).
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Citations for Rat IL-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Interleukin-4 Aggravates LPS-Induced Striatal Neurodegeneration In Vivo via Oxidative Stress and Polarization of Microglia/Macrophages
Authors: J Jang, A Hong, Y Chung, B Jin
International Journal of Molecular Sciences, 2022;23(1):.
Sample Types: In Vivo
Interleukin-4 and Interleukin-13 Exacerbate Neurotoxicity of Prothrombin Kringle-2 in Cortex In Vivo via Oxidative Stress
Authors: JY Jeong, YC Chung, BK Jin
Int J Mol Sci, 2019;20(8):.
Sample Types: Whole Tissue
Endogenous expression of interleukin-4 regulates macrophage activation and confines cavity formation after traumatic spinal cord injury.
Authors: Lee SI, Jeong SR, Kang YM
J. Neurosci. Res., 2010;88(11):2409-19.
Sample Types: In Vivo
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