Detects rat IL-5 in direct ELISAs. In direct ELISAs, 50% cross-reactivity with mouse IL-5 is observed and no cross‑reactivity with human, equine, canine, feline, or rhesus monkey IL-5 is observed.
Monoclonal Mouse IgG2B Clone # 299257
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat IL-5 Met20-Val132 Accession # Q08125
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.02-0.08 µg/mL in the presence of 1.25 ng/mL Recombinant Rat IL‑5.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑5 and Neutralization by Rat IL‑5 Antibody.
Recombinant Rat IL‑5 (Catalog # 545-RL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑5 Monoclonal Antibody (Catalog # MAB5451). The ND50 is typically 0.02‑0.08 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(IL-5) is a secreted glycoprotein that belongs to the alpha -helical group
of cytokines (1 ‑ 3). Unlike other family members, it is present as a
covalently linked antiparallel dimer (4, 5). The cDNA for rat IL-5
encodes a signal peptide and a 113 amino acid (aa) secreted mature
protein. Mature rat IL-5 shares 70%, 94%, 56%, 63%, 59% and 59%, aa
sequence identity with human, mouse, canine, equine, feline and porcine
IL-5, respectively. IL-5 is primarily produced by CD4+ Th2
cells, but also by activated eosinophils, mast cells, EBV-transformed B
cells, Reed-Sternberg cells in Hodgkin’s disease, and IL-2-stimulated
invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases
production and mobilization of eosinophils and CD34+
progenitors from the bone marrow and causes maturation of eosinophil
precursors outside the bone marrow (1, 6, 9, 10). The receptor for human
IL-5, mainly expressed by eosinophils, but also found on basophils and
mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a
shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds
IL-5 at low affinity, then associates with preformed beta c dimers, forming a
high-affinity receptor (12). IL-5 also binds proteoglycans, potentially
enhancing its activity (13). Soluble forms of IL-5 R alpha antagonize IL-5
and can be found in vivo (10, 14). In humans, IL-5 primarily
affects cells of the eosinophilic lineage, and promotes their
differentiation, maturation, activation, migration and survival, while
in mice IL-5 also enhances Ig class switching and release from B1 cells
(1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils
and primes them for histamine and leukotriene release (17).
Rosenberg, H. F. et al. (2007)
J. Allergy Clin. Immunol.
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M. et al. (2008) J. Biol. Chem.
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Horikawa, K. and
K. Takatsu (2006) Immunology
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