Rat Jagged 1 Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat Jagged 1 in ELISAs and Western blots. In sandwich ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Jagged 1 is observed, and less than 0.3% cross-reactivity with rhJagged 2 and recombinant mouse Jagged 2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat Jagged 1
    Ser32-Asp1068 (Gly57-Arg59 del, Asp63Thr, Arg64Leu, and Val-Arg-Pro-Tyr ins before Lys69)
    Accession # Q63722
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG)
    under non-reducing conditions only
  • Flow Cytometry
    2.5 µg/106 cells
    Rat cortical stem cells
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Rat Jagged 1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Rat Jagged 1 Antibody (Catalog # AF599)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Rat Jagged 1 Biotinylated Antibody (Catalog # BAF599)
  • ELISA Standard
     
    Recombinant Rat Jagged 1 Fc Chimera Protein, CF (Catalog # 599-JG)
  • Neutralization
    Measured by its ability to neutralize Jagged 1-induced differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 3 µg/mL Recombinant Rat Jagged 1 Fc Chimera and 150 ng/mL Recombinant Human BMP‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Differentiation Induced by Jagged 1 and Neutralization by Rat Jagged 1 Antibody. Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP‑2 (Catalog # 355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP‑2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND50 is typically 3-15 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jagged 1

Rat Jagged 1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged 1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged 1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged 1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged 1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged 1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged 1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged 1 shows 98% and 99% aa identity to human and mouse Jagged 1 extracellular domains respectively. Relative to the extracellular region of rat Jagged 2, the aa identity is 58%.

  • References:
    1. Lindsell, C.E. et al. (1995) Cell 80:909.
    2. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
    3. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
    4. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
    5. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
    6. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
    7. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.
  • Entrez Gene IDs:
    182 (Human); 29146 (Rat)
  • Alternate Names:
    AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Applications
Sample Type
  1. PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia
    Nat Commun, 2016;7(0):13650.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates.
    Authors: Hatakeyama J, Wakamatsu Y, Nagafuchi A, Kageyama R, Shigemoto R, Shimamura K
    Development, 2014;141(8):1671-82.
  3. Roles of Pofut1 and O-fucose in mammalian Notch signaling.
    Authors: Stahl M, Uemura K, Ge C, Shi S, Tashima Y, Stanley P
    J. Biol. Chem., 2008;283(20):13638-51.
    Species: Rat
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Rat Jagged 1 Antibody AF599
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 ICC/IF Mouse Anonymous 04/22/2016
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Immunocytochemistry/Immunofluorescence Rat Jagged 1 Antibody AF599
Immunocytochemistry/Immunofluorescence: Rat Jagged 1 Antibody [AF599]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedEmbryonic kidney
SpeciesMouse

Other Experimental Details

Other Experimental DetailsJag1 (Red)Six2 (Green)

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