Bovine IL-6 DuoSet ELISA

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Bovine IL-6 ELISA Standard Curve
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Product Details
Citations (12)
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Bovine IL-6 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Bovine IL-6 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells (2, 11-33). IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids (2). 

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (39). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (40). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130- expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 41). 
IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis (2, 5). IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity (1). It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis (1, 2). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of memory B cells and plasma cells (42).

Long Name:
Interleukin 6
Entrez Gene IDs:
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names:
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Citations for Bovine IL-6 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Anti-inflammatory effects of the prostaglandin D2/prostaglandin DP1 receptor and lipocalin-type prostaglandin D2 synthase/prostaglandin D2 pathways in bacteria-induced bovine endometrial tissue
    Authors: J Wu, F Bai, W Mao, B Liu, X Yang, J Zhang, T Li, G Borjigin, J Cao
    Veterinary research, 2022;53(1):98.
    Species: Bovine
    Sample Types: Tissue Homogenates
  2. Metformin activated AMPK signaling contributes to the alleviation of LPS-induced inflammatory responses in bovine mammary epithelial cells
    Authors: T Xu, X Wu, X Lu, Y Liang, Y Mao, JJ Loor, Z Yang
    Bmc Veterinary Research, 2021;17(1):97.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  3. Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves
    Authors: MG Marrero, B Dado-Senn, SL Field, G Yang, JP Driver, J Laporta
    PLoS ONE, 2021;16(6):e0252474.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  4. Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows
    Authors: L Beuckelaer, A De Vissche, FN Souza, E Meyer, F Haesebrouc, S Piepers, S De Vlieghe
    Veterinary research, 2021;52(1):137.
    Species: Bovine
    Sample Types: Whey
  5. Early inflammatory events of mastitis-a pilot study with the isolated perfused bovine udder
    Authors: KS Brand, V Filor, W Bäumer
    Bmc Veterinary Research, 2021;17(1):356.
    Species: Bovine
    Sample Types: Tissue Homogenates
  6. Intramammary treatment using allogeneic pure platelet-rich plasma in cows with subclinical mastitis caused by Gram-positive bacteria
    Authors: PC Duque-Madr, J Velasco-Bo, A Ceballos-M, C López, JU Carmona
    Scientific Reports, 2021;11(1):23737.
    Species: Bovine
    Sample Types: Milk
  7. TLR2/4 promotes PGE2 production to increase tissue damage in Escherichia coli-infected bovine endometrial explants via MyD88/p38 MAPK pathway
    Authors: T Li, L Hai, B Liu, W Mao, K Liu, Yuan Shen, Q Li, Y Guo, Y Jia, H Bao, J Cao
    Theriogenology, 2020;152(0):129-138.
    Species: Bovine
    Sample Types: Cell Culture Superantes
  8. Effects of omega-3 fatty acids on immune, health and growth variables in veal calves
    Authors: C Masmeijer, K van Leenen, L De Cremer, P Deprez, E Cox, B Devriendt, B Pardon
    Prev. Vet. Med., 2020;179(0):104979.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  9. Non-specific protection from respiratory tract infections in cattle generated by intranasal administration of an innate immune stimulant
    Authors: W Wheat, L Chow, V Rozo, J Herman, K Still Broo, A Colbath, R Hunter, S Dow
    PLoS ONE, 2020;15(6):e0235422.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  10. LP induced/mediated PGE2 synthesis through activation of the ERK/NF-?B pathway contributes to inflammatory damage triggered by Escherichia coli-infection in bovine endometrial tissue
    Authors: T Li, W Mao, B Liu, R Gao, S Zhang, J Wu, C Fu, Y Deng, K Liu, Y Shen, J Cao
    Vet. Microbiol., 2019;232(0):96-104.
    Species: Bovine
    Sample Types: Tissue Culture Supernates
  11. Jugular arginine infusion relieves lipopolysaccharide-triggered inflammatory stress and improves immunity status of lactating dairy cows
    Authors: FF Zhao, TY Wu, HR Wang, LY Ding, G Ahmed, HW Li, W Tian, YZ Shen
    J. Dairy Sci., 2018;0(0):.
    Species: Bovine
    Sample Types: Serum
  12. Regulation of cytokine gene expression by orosomucoid in neonatal swine adipose tissue
    Authors: TG Ramsay, MJ Stoll, le A Blomberg, TJ Caperna
    J Anim Sci Biotechnol, 2016;7(0):25.
    Species: Bovine
    Sample Types: Cell Culture Supernates


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