Detects cotton rat IFN‑ gamma in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant canine IFN-gamma, recombinant feline IFN-gamma, and recombinant human IFN-gamma, recombinant mouse IFN-gamma, recombinant rat IFN-gamma, and recombinant porcine IFN-gamma is observed.
Polyclonal Goat IgG
E. coli-derived recombinant cotton rat IFN-gamma His21-Ile170 Accession # Q9QXX2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG)
Cotton Rat IFN‑ gamma Biotinylated Antibody (Catalog # BAF565)
Recombinant Cotton Rat IFN-gamma Protein (Catalog # 565-RG)
Measured by its ability to neutralize IFN‑ gamma inhibition of VSV-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 20 ng/mL Recombinant Cotton Rat IFN‑ gamma.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN‑ gamma Inhibition of VSV-induced Cytopathy and Neutralization by Cotton Rat IFN‑ gamma Antibody.
Recombinant Cotton Rat IFN‑ gamma (Catalog # 565-RG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of VSV activity elicited by Recombinant Cotton Rat IFN‑ gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565). The ND50 is typically 0.3-1.2 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature cotton rat IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 43%‑50% amino acid sequence identity with bovine, canine, equine, feline, human, mouse, rat, rhesus, and porcine IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
Pestka, S. et al. (2004) Immunol. Rev. 202:8.
Houard, S. et al. (1999) Gene 240:107.
Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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