HIV-1 Gag p24 Antibody Summary
Accession # Q77YG1_9HIV1
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73601).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the HIV-1 Gag p24 DuoSet ELISA Kit (Catalog # DY7360-05) for convenient development of a sandwich ELISA or the HIV-1 Gag p24 Quantikine ELISA Kit (Catalog # DHP240) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of HIV‑1 Gag p24 by Western Blot. Western blot shows lysates of recombinant HIV-1 Gag p24. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73602) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIV‑1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Viral HIV‑1 Gag p24 ELISA Standard Curve. Recombinant Viral HIV‑1 Gag p24 protein was serially diluted 2-fold and captured by Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIV-1 Gag p24
The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
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