HIV-1 Gag p24 Antibody

Catalog # Availability Size / Price Qty
MAB73601-100
MAB73601-SP
Detection  of  HIV‑1 Gag p24 by Western  Blot.
2 Images
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HIV-1 Gag p24 Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral HIV-1 Gag p24 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 749138
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant viral HIV-1 Gag p24
Pro149-Gly354
Accession # Q77YG1_9HIV1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73602).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the HIV-1 Gag p24 DuoSet ELISA Kit (Catalog # DY7360-05) for convenient development of a sandwich ELISA or the HIV-1 Gag p24 Quantikine ELISA Kit (Catalog # DHP240) for a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of  HIV‑1 Gag p24 by Western  Blot. View Larger

Detection of HIV‑1 Gag p24 by Western Blot. Western blot shows lysates of recombinant HIV-1 Gag p24. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti- HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIV‑1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Viral HIV‑1 Gag p24 ELISA Standard Curve. Recombinant Viral HIV‑1 Gag p24 protein was serially diluted 2-fold and captured by Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Entrez Gene IDs
155030 (Viral)
Alternate Names
CA; Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag)

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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