HIV-1 Gag p24 Antibody

Name: HIV-1 Gag p24 Antibody
Brand: R&D Systems
SKU: MAB73601-SP
Rating: 5 (1 reviews)

Catalog #: MAB73601 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB73601-100
MAB73601-SP

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Detection of HIV‑1 Gag p24 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

HIV-1 Gag p24 Antibody Summary

Species Reactivity

Viral

Specificity

Detects viral HIV-1 Gag p24 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 749138

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant viral HIV-1 Gag p24
Pro149-Gly354
Accession # Q77YG1_9HIV1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73602).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the HIV-1 Gag p24 DuoSet ELISA Kit (Catalog # DY7360-05) for convenient development of a sandwich ELISA or the HIV-1 Gag p24 Quantikine ELISA Kit (Catalog # DHP240) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of HIV‑1 Gag p24 by Western Blot. Western blot shows lysates of recombinant HIV-1 Gag p24. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti- HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIV-1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ELISA

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Viral HIV‑1 Gag p24 ELISA Standard Curve. Recombinant Viral HIV-1 Gag p24 protein was serially diluted 2-fold and captured by Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Entrez Gene IDs

155030 (Viral)

Alternate Names

CA; Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag)