Key Product Details

Species Reactivity

Validated:

Human, Rat, Hamster

Cited:

Human, Mouse, Hamster

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Blocking/Neutralizing, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 171608
View all ACE-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740 (predicted)
Accession # Q9BYF1

Specificity

Detects recombinant human ACE-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ACE-1 or recombinant mouse ACE-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Rat/Hamster ACE‑2 Antibody

Detection of Human ACE-2 antibody by Western Blot.

Detection of Human ACE‑2 by Western Blot.

Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB9331) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ACE‑2 antibody in Rat Kidney by Immunohistochemistry (IHC-Fr).

ACE‑2 in Rat Kidney.

ACE‑2 was detected in immersion fixed frozen sections of rat kidney using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9331) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
ACE‑2 antibody in Hamster Lung by Immunohistochemistry (IHC-P).

ACE‑2 in Hamster Lung.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9331) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Rat/Hamster ACE‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Frozen sections of rat kidney and paraffin-embedded sections of hamster lung.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ACE‑2 (Catalog # 933-ZN), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: NS0 mouse myeloma cell line and human kidney tissue

Reviewed Applications

Read 1 review rated 5 using MAB9331 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function. ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2.

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Entrez Gene IDs

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Gene Symbol

ACE2

UniProt

Q9BYF1

Additional ACE-2 Products

Product Documents for Human/Rat/Hamster ACE‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat/Hamster ACE‑2 Antibody

For research use only

Citations for Human/Rat/Hamster ACE‑2 Antibody

Customer Reviews for Human/Rat/Hamster ACE‑2 Antibody (1)

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  • Human/Rat/Hamster ACE-2 Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Proximal tubules (kidney)
    Species: Human
    Verified Customer | Posted 08/31/2021
    Human/Rat/Hamster ACE‑2 Antibody MAB9331

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Protocols

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