Detection of Human, Mouse, and Rat BLMH/|
Bleomycin Hydrolase by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, mouse pancreas tissue, and rat pancreas tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human BLMH/Bleomycin Hydrolase Monoclonal Antibody (Catalog # MAB6200) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for BLMH/Bleomycin Hydrolase at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
|BLMH/Bleomycin Hydrolase in Human Testis. BLMH/Bleomycin Hydrolase was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human BLMH/Bleomycin Hydrolase Monoclonal Antibody (Catalog # MAB6200) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
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