Human Calcitonin R Antibody

Catalog # Availability Size / Price Qty
MAB46141-100
MAB46141-SP
Human Calcitonin R Antibody in Western Blot
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human Calcitonin R Antibody Summary

Species Reactivity
Human
Specificity
Detects human Calcitonin R in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 462815
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human Calcitonin R
Ala25-Ala474
Accession # NP_001733
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human Calcitonin R by Western Blot. View Larger

Detection of Human Calcitonin R by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line mock transfected or tranfected with Calcitonin R. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB46141) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Calcitonin R at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Calcitonin  R in Human Pancreatic Cancer Tissue. View Larger

Calcitonin R in Human Pancreatic Cancer Tissue. Calcitonin R was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB46141) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcitonin R

Calcitonin receptor (CALCR) is a glycosylated 70 kDa seven-transmembrane G protein-coupled receptor that mediates the hypocalcemic effects of the peptide hormone, calcitonin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal calcium excretion. CALCR polymorphisms and mutations have been associated with several bone disorders. Alternative splicing results in the deletion of 16 aa in the first cytoplasmic loop or 23 aa in the first extracellular region. Human CALCR shares 70%‑72% aa sequence identity with mouse and rat CACLR.

Long Name
Calcitonin Receptor
Entrez Gene IDs
799 (Human); 12311 (Mouse); 116506 (Rat)
Alternate Names
Calcitonin R; calcitonin receptor; CalcitoninR; CALCR; CRT; CTR; CT-R; CTR1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Calcitonin R Antibody

There are currently no reviews for this product. Be the first to review Human Calcitonin R Antibody and earn rewards!

Have you used Human Calcitonin R Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review