Human CCL17/TARC Antibody

(8 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TARC is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CCL17/TARC (R&D Systems, Catalog # 364-DN)
    Ala24-Ser94
    Accession # Q92583
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human CCL17/TARC (Catalog # 364-DN)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL17/TARC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody. Recombinant Human CCL17/TARC (Catalog #
364‑DN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL17/
TARC (0.01 µg/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364). The ND50 is typically 0.3-1.5 µg/mL.

Immunohistochemistry
CCL17/TARC in Human Spleen. CCL17/TARC was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL17/TARC
CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.
  • References:
    1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
    2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
    3. Nomiyama, H. et al. (1997) Genomics 40:211.
  • Entrez Gene IDs:
    6361 (Human); 20295 (Mouse)
  • Alternate Names:
    ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Pathogenesis of cBFL in common with IPF? Correlation of IP-10/TARC ratio with histological patterns.
    Authors: Kishi M, Miyazaki Y, Jinta T, Furusawa H, Ohtani Y, Inase N, Yoshizawa Y
    Thorax, 2008;63(9):810-6.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
    Authors: Heijink IH, Kies PM, Kauffman HF, Postma DS, van Oosterhout AJ, Vellenga E
    J. Immunol., 2007;178(12):7678-85.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
    Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
    Blood, 2007;111(4):2339-46.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Gene expression analysis of dendritic/Langerhans cells and Langerhans cell histiocytosis.
    Authors: Rust R, Kluiver J, Visser L, Harms G, Blokzijl T, Kamps W, Poppema S, Van Den Berg A
    J. Pathol., 2006;209(4):474-83.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  6. Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
    Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
    Int. J. Oncol., 2004;25(3):605-13.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. TARC augments TNF-alpha-induced CTACK production in keratinocytes.
    Authors: Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M
    Exp. Dermatol., 2004;13(9):551-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  8. Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas.
    Authors: Maggio EM, Van Den Berg A, Visser L, Diepstra A, Kluiver J, Emmens R, Poppema S
    Int. J. Cancer, 2002;99(5):665-72.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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