Human CCL19/MIP-3 beta Antibody

Catalog # Availability Size / Price Qty
MAB361-SP
MAB361-100

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CCL19/MIP‑3 beta  in Human Tonsil.
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Product Details
Citations (7)
FAQs
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Reviews (1)

Human CCL19/MIP-3 beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL19/MIP-3 beta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 25, recombinant mouse CCL2, 3, 4, 5, 6, 7, 9, 11, 12, 21, 22, or 25 is observed.
Source
Monoclonal Mouse IgG2B Clone # 54909
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL19/MIP-3 beta
Gly22-Ser98
Accession # Q99731.1
Formulation
Supplied as a 0.2 μm filtered solution in Tris and Acetic Acid. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL19/MIP‑3 beta (Catalog # 361-MI)
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry CCL19/MIP‑3 beta  antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

CCL19/MIP‑3 beta in Human Tonsil. CCL19/MIP-3 beta was detected in immersion fixed paraffin-embedded sections of human tonsil using 25 µg/mL Mouse Anti-Human CCL19/ MIP-3 beta Monoclonal Antibody (Catalog # MAB361) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry CCL19/MIP‑3 beta  antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CCL19/MIP‑3 beta in Human PBMCs. CCL19/MIP-3 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CCL19/MIP-3 beta Monoclonal Anti-body (Catalog # MAB361) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry Detection of CCL19/MIP-3 beta  antibody in Human Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of CCL19/MIP‑3 beta in Human Dendritic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human CCL19/MIP-3 beta Monoclonal Antibody (Catalog # MAB361, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: CCL19/MIP-3 beta

CCL19, also known as MIP-3 beta and ELC (EBI1-Ligand Chemokine), is a 77 amino acid (aa) beta chemokine that is distantly related to other beta chemokines (20-30% aa sequence identity). The gene for MIP-3 beta has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta is constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is
down‑regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta is chemotactic for cultured human lymphocytes. MIP-3 beta is a ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

References
  1. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Entrez Gene IDs
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

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Citations for Human CCL19/MIP-3 beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. A Versatile Toolkit for Semi-Automated Production of Fluorescent Chemokines to Study CCR7 Expression and Functions
    Authors: M Artinger, C Matti, OJ Gerken, CT Veldkamp, DF Legler
    International Journal of Molecular Sciences, 2021;22(8):.
    Species: Human
    Sample Types: Protein Lysate
    Applications: Western Blot
  2. Induction of immunoregulatory CD271+ cells by metastatic tumor cells that express human endogenous retrovirus H.
    Authors: Kudo-Saito C, Yura M, Yamamoto R, Kawakami Y
    Cancer Res, 2014;74(5):1361-70.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014;14(0):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia.
    Authors: Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R
    Ann. Neurol., 2009;66(4):521-31.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  5. Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
    Authors: Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
    J. Immunol., 2006;176(10):5735-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Expression of macrophage inflammatory protein-3 beta/CCL19 in pulmonary sarcoidosis.
    Authors: Gibejova A, Mrazek F, Subrtova D, Sekerova V, Szotkowska J, Kolek V, du Bois RM, Petrek M
    Am. J. Respir. Crit. Care Med., 2003;167(12):1695-703.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
    Authors: Rump L, Mattey D, Kehoe O, Middleton J
    Cytokine, 0;97(0):133-140.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Human CCL19/MIP-3 beta Antibody
By Anonymous on 03/13/2022
Application: IHC Sample Tested: Tonsil tissue Species: Human