|CCL19/MIP‑3 beta in Human PBMCs. CCL19/MIP‑3 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CCL19/MIP‑3 beta Monoclonal Antibody (Catalog # MAB361) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
CCL19/MIP‑3 beta in Human Tonsil. CCL19/MIP‑3 beta was detected in immersion fixed paraffin-embedded sections of human tonsil using 25 µg/mL Mouse Anti-Human CCL19/|
MIP‑3 beta Monoclonal Antibody (Catalog # MAB361) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Intracellular Staining by Flow Cytometry
Detection of CCL19/MIP‑3 beta in Human Dendritic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human CCL19/MIP‑3 beta Monoclonal Antibody (Catalog # MAB361, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody |
(Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I
(Catalog # FC005).
CCL19, also known as MIP-3 beta and ELC (EBI1-Ligand Chemokine), is a 77 amino acid (aa) beta chemokine that is distantly related to other beta chemokines (20-30% aa sequence identity). The gene for MIP-3 beta has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta is constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is
down‑regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta is chemotactic for cultured human lymphocytes. MIP-3 beta is a ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.
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