Human CCL2/JE/MCP-1 Antibody Summary
Accession # P13500
Human CCL2/JE/MCP-1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human CCL2/JE/MCP‑1 ELISA Standard Curve. Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC) was serially diluted 2-fold and captured by Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB679R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF279) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP-1 Antibody. Recombinant Human CCL2/MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679R). The ND50 is typically 0.5-2.0 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.
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After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC).
Paired with SulfoTag-modified AF-279-NA as a detection antibody. Calibration curves with Recombinant Human CCL2 (279-MC-010/CF) is shown (dynamic range 6-25,000 pg/ml)