Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 23007R
View all CCL2/JE/MCP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL2/JE/MCP‑1
Gln24-Thr99
Accession # P13500

Specificity

Detects human CCL2/JE/MCP‑1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL2/JE/MCP‑1 Antibody

Human CCL2/JE/MCP-1 Antibody in ELISA Standard Curve.

Human CCL2/JE/MCP‑1 ELISA Standard Curve.

Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC) was serially diluted 2-fold and captured by Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF279) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP-1 Antibody.

Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP-1 Antibody.

Recombinant Human CCL2/MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679R). The ND50 is typically 0.5-2.0 µg/mL.

Applications for Human CCL2/JE/MCP‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC)

Neutralization

Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 75 ng/mL Recombinant Human CCL2/JE/MCP‑1.

Human CCL2/JE/MCP-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog # BAF279)
  • Standard: Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB679R in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

UniProt

P13500

Additional CCL2/JE/MCP-1 Products

Product Documents for Human CCL2/JE/MCP‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL2/JE/MCP‑1 Antibody

For research use only

Customer Reviews for Human CCL2/JE/MCP‑1 Antibody (1)

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  • Human CCL2/JE/MCP-1 Antibody
    Name: Zoia Levashova
    Application: MSD assay
    Sample Tested: Vitreous humor
    Species: Cynomolgus Monkey
    Verified Customer | Posted 11/20/2018
    After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC). Paired with SulfoTag-modified AF-279-NA as a detection antibody. Calibration curves with Recombinant Human CCL2 (279-MC-010/CF) is shown (dynamic range 6-25,000 pg/ml)
    Human CCL2/JE/MCP‑1 Antibody MAB679R

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