Human CCL2/JE/MCP-1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB679R-100
MAB679R-SP
Human  CCL2/JE/MCP‑1 ELISA Standard Curve.
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Human CCL2/JE/MCP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL2/JE/MCP‑1 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2B Clone # 23007R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human CCL2/JE/MCP‑1
Gln24-Thr99
Accession # P13500
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC)

Human CCL2/JE/MCP-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog # BAF279)

Standard: Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC)

Neutralization
Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 75 ng/mL Recombinant Human CCL2/JE/MCP‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

ELISA Capture (Matched Antibody Pair) Human  CCL2/JE/MCP‑1 ELISA Standard Curve. View Larger

Human CCL2/JE/MCP‑1 ELISA Standard Curve. Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC) was serially diluted 2-fold and captured by Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB679R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF279) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Neutralization Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP-1 Antibody. View Larger

Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP-1 Antibody. Recombinant Human CCL2/MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679R). The ND50 is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.

Entrez Gene IDs
6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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