Human CD4 Antibody

Name: Human CD4 Antibody
Brand: R&D Systems
SKU: MAB114341
Price: 419 USD
Availability: InStock

R&D Systems | Catalog # MAB114341

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 1068606

View all CD4 Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human CD4
Gln26-Lys463
Accession # P08575

Specificity

Detects human CD4 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human CD4 Antibody

Detection of CD4 in PBMC lymphocytes stained with CD4 RPA-T4 by Flow Cytometry.

PBMC lymphocytes were stained with CD4 RPA-T4 (MAB37912). Cells were washed and stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog #MAB114341) or (B) Mouse IgG2B Isotype Control (MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC conjugated Monoclonal Antibody (FAB100A). This demonstrates non-competing epitopes of MAB114341 and MAB37912 which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins.

Detection of CD4 in Human peripheral blood lymphocytes by Flow Cytometry

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB114341) which was PE-conjugated (FAB114341P), and Mouse Anti-Human CD4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (FAB37912R). This demonstrates non-competing epitopes of the two clones, which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD4 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: PBMC Lymphocytes

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) precursor protein with a 25 aa signal peptide, a 371 aa extracellular region containing four immunoglobulin homology domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.