Human CD43 Antibody

Catalog # Availability Size / Price Qty
Detection of CD43 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (1)
Supplemental Products
Reviews (2)

Human CD43 Antibody Summary

Species Reactivity
Detects human CD43. Stains human CD43-transfected cells but not irrelevant transfectants.
Monoclonal Mouse IgG2B Clone # 290111
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human CD43
Accession # P16150
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD43 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD43 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD43 Monoclonal Antibody (Catalog # MAB2038) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody. View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD43 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD43 in Human PBMCs. CD43 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD43 Monoclonal Antibody (Catalog # MAB2038) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD43

CD43, also known as Leukosialin and Sialophorin and Ly-48 in mouse, is a 100-105 kDa type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells. Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific. CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion. Mature CD43 shows 49% amino (aa) sequence identity between human and mouse.

Long Name
Cluster of Differentiation 43
Entrez Gene IDs
6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)
Alternate Names
CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN

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Citation for Human CD43 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1alpha, IL-1beta, IL-12b, and CCL4 from differentiated HL-60 cells.
    Authors: Naegelen I, Plancon S, Nicot N, Kaoma T, Muller A, Vallar L, Tschirhart E, Brechard S
    J Leukoc Biol, 2014-12-29;97(3):557-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC


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Human CD43 Antibody
By Anonymous on 05/03/2023
Application: IP Sample Tested: K562 human chronic myelogenous leukemia cell line,KG-1a human acute myelogenous leukemia cell line Species: Human

Human CD43 Antibody
By Anonymous on 03/02/2016
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human