Human CD43 Antibody Summary
Accession # P16150
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD43 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD43 Monoclonal Antibody (Catalog # MAB2038) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody. View our protocol for Staining Membrane-associated Proteins.
CD43 in Human PBMCs. CD43 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD43 Monoclonal Antibody (Catalog # MAB2038) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD43, also known as Leukosialin and Sialophorin and Ly-48 in mouse, is a 100-105 kDa type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells. Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific. CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion. Mature CD43 shows 49% amino (aa) sequence identity between human and mouse.
Citation for Human CD43 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1alpha, IL-1beta, IL-12b, and CCL4 from differentiated HL-60 cells.
Authors: Naegelen I, Plancon S, Nicot N, Kaoma T, Muller A, Vallar L, Tschirhart E, Brechard S
J Leukoc Biol, 2015;97(3):557-71.
Sample Types: Whole Cells
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